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Skewed VH and V kappa gene family expression and pairing occurs among B lymphocytes in autoimmune motheaten mice.

Publication ,  Journal Article
Saitoh, Y; Kelsoe, G; Bona, C; Kaushik, A
Published in: Autoimmunity
1995

Motheaten mice homozygous for the recessive mev mutation develop a fatal immunodeficiency syndrome associated with hypergammaglobulinemia, thymic aplasia, production of autoantibodies and development of a severe lupus like systemic autoimmune disease. Most B lymphocytes in this mutant strain belong to B-1 subset. We have addressed the question if differences existed in the V-gene repertoire of autoimmune mev/mev mice as compared to phenotypically normal mev/+ and C57BL/6 background strain by examining the VH and V kappa gene family expression as well as the association of VH and V kappa gene families among B lymphocyte clones. The data outlined here demonstrate that both the expression of VH and V kappa gene families and their association is skewed in mev/mev mice, suffering from systemic autoimmune disease, and differs significantly from phenotypically normal mev/+ litter mates as well as the C57BL/6 background strain. In addition, VH+V kappa gene family pairs in phenotypically normal mev/+ differed from normal C57BL/6 mice suggesting that motheaten mutation, whether homozygous or heterozygous, alters the development of the B lymphocyte repertoire. These observations suggest positive selection of B-1 lymphocytes in autoimmune motheaten mice either as a result of selective processes, via receptor-ligand interactions, operating on the development of the primary antibody repertoire or defective B lymphocyte haematopoiesis due to the deficiency of haematopoietic cell phosphatase involved in determining the threshold by which B cells respond to self antigen(s).

Duke Scholars

Published In

Autoimmunity

DOI

ISSN

0891-6934

Publication Date

1995

Volume

21

Issue

3

Start / End Page

185 / 193

Location

England

Related Subject Headings

  • Recombinant Proteins
  • Multigene Family
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Lipopolysaccharides
  • Interleukin-4
  • Immunology
  • Immunoglobulin kappa-Chains
 

Citation

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Saitoh, Y., Kelsoe, G., Bona, C., & Kaushik, A. (1995). Skewed VH and V kappa gene family expression and pairing occurs among B lymphocytes in autoimmune motheaten mice. Autoimmunity, 21(3), 185–193. https://doi.org/10.3109/08916939509008015
Saitoh, Y., G. Kelsoe, C. Bona, and A. Kaushik. “Skewed VH and V kappa gene family expression and pairing occurs among B lymphocytes in autoimmune motheaten mice.Autoimmunity 21, no. 3 (1995): 185–93. https://doi.org/10.3109/08916939509008015.
Saitoh, Y., et al. “Skewed VH and V kappa gene family expression and pairing occurs among B lymphocytes in autoimmune motheaten mice.Autoimmunity, vol. 21, no. 3, 1995, pp. 185–93. Pubmed, doi:10.3109/08916939509008015.
Journal cover image

Published In

Autoimmunity

DOI

ISSN

0891-6934

Publication Date

1995

Volume

21

Issue

3

Start / End Page

185 / 193

Location

England

Related Subject Headings

  • Recombinant Proteins
  • Multigene Family
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Lipopolysaccharides
  • Interleukin-4
  • Immunology
  • Immunoglobulin kappa-Chains