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Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.

Publication ,  Journal Article
Schulze, DH; Kelsoe, G
Published in: J Exp Med
July 1, 1987

The filter paper disc method for cloning inducible lymphocytes was used to census the splenic B cell population of C57BL/6 and BALB/c mice for the expression of three VH gene-families, VH X-24, -Q52, and -J558. B cell colonies, arising from single founder lymphocytes, were identified by in situ hybridization with VH family- and C mu-specific cDNA probes. Some 6.7 X 10(4) C mu+ colonies were screened. Among C57BL/6- or BALB/c-derived colonies, approximately 3% were VH X-24+, approximately 19% were VH Q52+, and approximately 54% were VH J558+. These frequencies are consistent with a process of equiprobable expression for individual VH segments, and provide direct evidence that normal splenic B lymphocytes use a process of random genetic combinatorics to generate the antibody repertoire.

Duke Scholars

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

July 1, 1987

Volume

166

Issue

1

Start / End Page

163 / 172

Location

United States

Related Subject Headings

  • Spleen
  • Nucleic Acid Hybridization
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Lipopolysaccharides
  • Immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Genotype
 

Citation

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Schulze, D. H., & Kelsoe, G. (1987). Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice. J Exp Med, 166(1), 163–172. https://doi.org/10.1084/jem.166.1.163
Schulze, D. H., and G. Kelsoe. “Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.J Exp Med 166, no. 1 (July 1, 1987): 163–72. https://doi.org/10.1084/jem.166.1.163.
Schulze, D. H., and G. Kelsoe. “Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.J Exp Med, vol. 166, no. 1, July 1987, pp. 163–72. Pubmed, doi:10.1084/jem.166.1.163.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

July 1, 1987

Volume

166

Issue

1

Start / End Page

163 / 172

Location

United States

Related Subject Headings

  • Spleen
  • Nucleic Acid Hybridization
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Lipopolysaccharides
  • Immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Genotype