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Regulation of the B cell response to T-dependent antigens by classical pathway complement.

Publication ,  Journal Article
Fischer, MB; Ma, M; Goerg, S; Zhou, X; Xia, J; Finco, O; Han, S; Kelsoe, G; Howard, RG; Rothstein, TL; Kremmer, E; Rosen, FS; Carroll, MC
Published in: J Immunol
July 15, 1996

Mice deficient in complement components C3 (C3 -/-) and C4 (C4 -/-) were found to have a profound defect in their Ab response to a T-dependent Ag (bacteriophage (phi X174). Characterization of the deficient mice demonstrated a diminished level of peanut agglutinin+ germinal centers and a failure in isotype switching despite normal B cell signaling in vitro. The nature of the defect was found to lie at the B cell level, as the T cells were primed in C3- and C4-deficient mice as well as those in wild-type mice. These results, and the finding that the defect could be partly reversed by a 10-fold increase in Ag dose, support the hypothesis that covalent attachment of complement ligands, i.e., C3b and C3d to the Ag-Ab complex, increases its immunogenicity.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1996

Volume

157

Issue

2

Start / End Page

549 / 556

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Germinal Center
  • Female
 

Citation

APA
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ICMJE
MLA
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Fischer, M. B., Ma, M., Goerg, S., Zhou, X., Xia, J., Finco, O., … Carroll, M. C. (1996). Regulation of the B cell response to T-dependent antigens by classical pathway complement. J Immunol, 157(2), 549–556.
Fischer, M. B., M. Ma, S. Goerg, X. Zhou, J. Xia, O. Finco, S. Han, et al. “Regulation of the B cell response to T-dependent antigens by classical pathway complement.J Immunol 157, no. 2 (July 15, 1996): 549–56.
Fischer MB, Ma M, Goerg S, Zhou X, Xia J, Finco O, et al. Regulation of the B cell response to T-dependent antigens by classical pathway complement. J Immunol. 1996 Jul 15;157(2):549–56.
Fischer, M. B., et al. “Regulation of the B cell response to T-dependent antigens by classical pathway complement.J Immunol, vol. 157, no. 2, July 1996, pp. 549–56.
Fischer MB, Ma M, Goerg S, Zhou X, Xia J, Finco O, Han S, Kelsoe G, Howard RG, Rothstein TL, Kremmer E, Rosen FS, Carroll MC. Regulation of the B cell response to T-dependent antigens by classical pathway complement. J Immunol. 1996 Jul 15;157(2):549–556.

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1996

Volume

157

Issue

2

Start / End Page

549 / 556

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Germinal Center
  • Female