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Regulation of the B cell response to T-dependent antigens by classical pathway complement.

Publication ,  Journal Article
Fischer, MB; Ma, M; Goerg, S; Zhou, X; Xia, J; Finco, O; Han, S; Kelsoe, G; Howard, RG; Rothstein, TL; Kremmer, E; Rosen, FS; Carroll, MC
Published in: J Immunol
July 15, 1996

Mice deficient in complement components C3 (C3 -/-) and C4 (C4 -/-) were found to have a profound defect in their Ab response to a T-dependent Ag (bacteriophage (phi X174). Characterization of the deficient mice demonstrated a diminished level of peanut agglutinin+ germinal centers and a failure in isotype switching despite normal B cell signaling in vitro. The nature of the defect was found to lie at the B cell level, as the T cells were primed in C3- and C4-deficient mice as well as those in wild-type mice. These results, and the finding that the defect could be partly reversed by a 10-fold increase in Ag dose, support the hypothesis that covalent attachment of complement ligands, i.e., C3b and C3d to the Ag-Ab complex, increases its immunogenicity.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1996

Volume

157

Issue

2

Start / End Page

549 / 556

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Germinal Center
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fischer, M. B., Ma, M., Goerg, S., Zhou, X., Xia, J., Finco, O., … Carroll, M. C. (1996). Regulation of the B cell response to T-dependent antigens by classical pathway complement. J Immunol, 157(2), 549–556.

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1996

Volume

157

Issue

2

Start / End Page

549 / 556

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Germinal Center
  • Female