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Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice.

Publication ,  Journal Article
Striebich, CC; Miceli, RM; Schulze, DH; Kelsoe, G; Cerny, J
Published in: J Immunol
March 1, 1990

LPS-stimulated B cells were used to generate a panel of mAb that were a random sample of the preimmune repertoire of C57BL/6 and highly autoimmune, viable motheaten mice. These mAb were tested for reactivity to a number of "self" and foreign Ag. Binding that could be detected only at nM mAb concentrations or less was considered significant. We found that a surprisingly high number of the mAb bound one or more of the Ag tested, and many mAb bound more than a single Ag. Ag-induced mAb were likewise tested and found to have greatly reduced cross-reactivities. We found no significant differences, either in frequency of Ag binding or degree of cross-reactivity, between normal and autoimmune mice. Furthermore, the frequency with which a given Ag was bound by our panel of mAb was found to be proportional to the size of the Ag. The frequency with which individual VH gene families were expressed by our panel was consistent with a stochastic usage of VH genes in the preimmune repertoire. We interpret these data as showing that the preimmune repertoire is highly cross-reactive and that the activation of autoreactive clones in autoimmune animals is due to a defect in cellular regulation rather than a difference in repertoire.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 1, 1990

Volume

144

Issue

5

Start / End Page

1857 / 1865

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Multigene Family
  • Molecular Weight
  • Mice, Mutant Strains
  • Mice
  • Immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Hybridomas
  • Genes, Immunoglobulin
 

Citation

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Striebich, C. C., Miceli, R. M., Schulze, D. H., Kelsoe, G., & Cerny, J. (1990). Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice. J Immunol, 144(5), 1857–1865.
Striebich, C. C., R. M. Miceli, D. H. Schulze, G. Kelsoe, and J. Cerny. “Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice.J Immunol 144, no. 5 (March 1, 1990): 1857–65.
Striebich CC, Miceli RM, Schulze DH, Kelsoe G, Cerny J. Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice. J Immunol. 1990 Mar 1;144(5):1857–65.
Striebich, C. C., et al. “Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice.J Immunol, vol. 144, no. 5, Mar. 1990, pp. 1857–65.
Striebich CC, Miceli RM, Schulze DH, Kelsoe G, Cerny J. Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice. J Immunol. 1990 Mar 1;144(5):1857–1865.

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 1, 1990

Volume

144

Issue

5

Start / End Page

1857 / 1865

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Multigene Family
  • Molecular Weight
  • Mice, Mutant Strains
  • Mice
  • Immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Hybridomas
  • Genes, Immunoglobulin