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The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development.

Publication ,  Journal Article
Liang, H-E; Hsu, L-Y; Cado, D; Cowell, LG; Kelsoe, G; Schlissel, MS
Published in: Immunity
November 2002

Previous in vitro studies defined the minimal regions of RAG1 and RAG2 essential for V(D)J recombination. In order to characterize the role of the C-terminal "dispensable" portion of RAG2, we generated core-RAG2 knock-in mice. We found that the core-RAG2-containing recombinase complex is selectively defective in catalyzing V-to-DJ rearrangement at the IgH and TCRbeta loci, resulting in partial developmental blocks in B and T lymphopoiesis. Analysis of recombination intermediates showed defects at the cleavage phase of the reaction. We also observed a reduction in overall recombinase activity in core-RAG2-expressing thymocytes, leading us to suggest that the interaction of a defective recombinase with RSS sequences unique to VH and Vbeta gene segments may underlie the specific V-to-DJ rearrangement defect in core-RAG2 mice.

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Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

November 2002

Volume

17

Issue

5

Start / End Page

639 / 651

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • T-Lymphocytes
  • Recombination, Genetic
  • Mice
  • Immunology
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • DNA-Binding Proteins
  • DNA Nucleotidyltransferases
  • Cell Line
 

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Liang, H.-E., Hsu, L.-Y., Cado, D., Cowell, L. G., Kelsoe, G., & Schlissel, M. S. (2002). The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development. Immunity, 17(5), 639–651. https://doi.org/10.1016/s1074-7613(02)00448-x
Liang, Hong-Erh, Lih-Yun Hsu, Dragana Cado, Lindsay G. Cowell, Garnett Kelsoe, and Mark S. Schlissel. “The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development.Immunity 17, no. 5 (November 2002): 639–51. https://doi.org/10.1016/s1074-7613(02)00448-x.
Liang H-E, Hsu L-Y, Cado D, Cowell LG, Kelsoe G, Schlissel MS. The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development. Immunity. 2002 Nov;17(5):639–51.
Liang, Hong-Erh, et al. “The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development.Immunity, vol. 17, no. 5, Nov. 2002, pp. 639–51. Pubmed, doi:10.1016/s1074-7613(02)00448-x.
Liang H-E, Hsu L-Y, Cado D, Cowell LG, Kelsoe G, Schlissel MS. The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development. Immunity. 2002 Nov;17(5):639–651.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

November 2002

Volume

17

Issue

5

Start / End Page

639 / 651

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • T-Lymphocytes
  • Recombination, Genetic
  • Mice
  • Immunology
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • DNA-Binding Proteins
  • DNA Nucleotidyltransferases
  • Cell Line