A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.
Publication
, Journal Article
Hanzlik, AJ; Osemlak-Hanzlik, MM; Hauser, MA; Kurnit, DM
Published in: Nat Genet
January 1993
To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
Duke Scholars
Published In
Nat Genet
DOI
ISSN
1061-4036
Publication Date
January 1993
Volume
3
Issue
1
Start / End Page
44 / 48
Location
United States
Related Subject Headings
- Transcription, Genetic
- Recombination, Genetic
- RNA-Binding Proteins
- Polymerase Chain Reaction
- Organ Specificity
- Nerve Tissue Proteins
- Molecular Sequence Data
- Humans
- Genetic Techniques
- Fragile X Syndrome
Citation
APA
Chicago
ICMJE
MLA
NLM
Hanzlik, A. J., Osemlak-Hanzlik, M. M., Hauser, M. A., & Kurnit, D. M. (1993). A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development. Nat Genet, 3(1), 44–48. https://doi.org/10.1038/ng0193-44
Hanzlik, A. J., M. M. Osemlak-Hanzlik, M. A. Hauser, and D. M. Kurnit. “A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.” Nat Genet 3, no. 1 (January 1993): 44–48. https://doi.org/10.1038/ng0193-44.
Hanzlik AJ, Osemlak-Hanzlik MM, Hauser MA, Kurnit DM. A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development. Nat Genet. 1993 Jan;3(1):44–8.
Hanzlik, A. J., et al. “A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.” Nat Genet, vol. 3, no. 1, Jan. 1993, pp. 44–48. Pubmed, doi:10.1038/ng0193-44.
Hanzlik AJ, Osemlak-Hanzlik MM, Hauser MA, Kurnit DM. A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development. Nat Genet. 1993 Jan;3(1):44–48.
Published In
Nat Genet
DOI
ISSN
1061-4036
Publication Date
January 1993
Volume
3
Issue
1
Start / End Page
44 / 48
Location
United States
Related Subject Headings
- Transcription, Genetic
- Recombination, Genetic
- RNA-Binding Proteins
- Polymerase Chain Reaction
- Organ Specificity
- Nerve Tissue Proteins
- Molecular Sequence Data
- Humans
- Genetic Techniques
- Fragile X Syndrome