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ATM binds to beta-adaptin in cytoplasmic vesicles.

Publication ,  Journal Article
Lim, DS; Kirsch, DG; Canman, CE; Ahn, JH; Ziv, Y; Newman, LS; Darnell, RB; Shiloh, Y; Kastan, MB
Published in: Proc Natl Acad Sci U S A
August 18, 1998
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Inherited mutations in the ATM gene lead to a complex clinical phenotype characterized by neuronal degeneration, oculocutaneous telangiectasias, immune dysfunction, and cancer predisposition. Using the yeast two-hybrid system, we demonstrate that ataxia telangiectasia mutated (ATM) binds to beta-adaptin, one of the components of the AP-2 adaptor complex, which is involved in clathrin-mediated endocytosis of receptors. The interaction between ATM and beta-adaptin was confirmed in vitro, and coimmunoprecipitation and colocalization studies show that the proteins also associate in vivo. ATM also interacts in vitro with beta-NAP, a neuronal-specific beta-adaptin homolog that was identified as an autoantigen in a patient with cerebellar degeneration. Our data describing the association of ATM with beta-adaptin in vesicles indicate that ATM may play a role in intracellular vesicle and/or protein transport mechanisms.

Duke Scholars

Michael Barry Kastan
Author Michael Barry Kastan Pharmacology & Cancer Biology
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.95.17.10146

ISSN

0027-8424

Publication Date

August 18, 1998

Volume

95

Issue

17

Start / End Page

10146 / 10151

Location

United States

Related Subject Headings

  • Vero Cells
  • Tumor Suppressor Proteins
  • Transfection
  • Saccharomyces cerevisiae
  • Proteins
  • Protein Serine-Threonine Kinases
  • Protein Binding
  • Phosphoproteins
  • Peptide Fragments
  • Nerve Tissue Proteins
 

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Lim, D. S., Kirsch, D. G., Canman, C. E., Ahn, J. H., Ziv, Y., Newman, L. S., … Kastan, M. B. (1998). ATM binds to beta-adaptin in cytoplasmic vesicles. Proc Natl Acad Sci U S A, 95(17), 10146–10151. https://doi.org/10.1073/pnas.95.17.10146
Lim, D. S., D. G. Kirsch, C. E. Canman, J. H. Ahn, Y. Ziv, L. S. Newman, R. B. Darnell, Y. Shiloh, and M. B. Kastan. “ATM binds to beta-adaptin in cytoplasmic vesicles.” Proc Natl Acad Sci U S A 95, no. 17 (August 18, 1998): 10146–51. https://doi.org/10.1073/pnas.95.17.10146.
Lim DS, Kirsch DG, Canman CE, Ahn JH, Ziv Y, Newman LS, et al. ATM binds to beta-adaptin in cytoplasmic vesicles. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10146–51.
Lim, D. S., et al. “ATM binds to beta-adaptin in cytoplasmic vesicles.” Proc Natl Acad Sci U S A, vol. 95, no. 17, Aug. 1998, pp. 10146–51. Pubmed, doi:10.1073/pnas.95.17.10146.
Lim DS, Kirsch DG, Canman CE, Ahn JH, Ziv Y, Newman LS, Darnell RB, Shiloh Y, Kastan MB. ATM binds to beta-adaptin in cytoplasmic vesicles. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10146–10151.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.95.17.10146

ISSN

0027-8424

Publication Date

August 18, 1998

Volume

95

Issue

17

Start / End Page

10146 / 10151

Location

United States

Related Subject Headings

  • Vero Cells
  • Tumor Suppressor Proteins
  • Transfection
  • Saccharomyces cerevisiae
  • Proteins
  • Protein Serine-Threonine Kinases
  • Protein Binding
  • Phosphoproteins
  • Peptide Fragments
  • Nerve Tissue Proteins