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Chlorpyrifos targets developing glia: effects on glial fibrillary acidic protein.

Publication ,  Journal Article
Garcia, SJ; Seidler, FJ; Qiao, D; Slotkin, TA
Published in: Brain Res Dev Brain Res
February 28, 2002

The organophosphate pesticide, chlorpyrifos (CPF), is a developmental neurotoxicant. In cell cultures, CPF affects gliotypic cells to a greater extent than neuronotypic cells, suggesting that glial development is a specific target. We administered CPF to developing rats and examined the levels of glial fibrillary acidic protein (GFAP), an astrocytic marker. Prenatal CPF exposure (gestational days 17-20) elicited an increase in GFAP levels in fetal brain, but the effect was seen only at high doses that elicited maternal and fetal systemic toxicity. Early postnatal (PN) CPF treatment (PN1-4) elicited effects only in the cerebellum of male rats; GFAP was suppressed initially (PN5) and showed a rebound elevation (PN10) before returning to normal values by PN30. In contrast, when we administered CPF during the peak of gliogenesis and glial cell differentiation (PN11-14), GFAP was initially decreased across all brain regions and in both sexes; in males, subsequent elevations were seen on PN30, with the largest effect in the striatum; females also showed an increase in striatal GFAP. Our results indicate that CPF disrupts the pattern of glial development in vivo, with the maximum effect corresponding to the peak period of gliogenesis and glial cell differentiation. As glia are responsible for axonal guidance, synaptogenesis and neuronal nutrition, glial targeting suggests that these late-occurring developmental processes are vulnerable to CPF, extending the critical period for susceptibility into stages of synaptic plasticity, myelination, and architectural modeling of the developing brain.

Duke Scholars

Published In

Brain Res Dev Brain Res

DOI

ISSN

0165-3806

Publication Date

February 28, 2002

Volume

133

Issue

2

Start / End Page

151 / 161

Location

Netherlands

Related Subject Headings

  • Sex Factors
  • Rats, Sprague-Dawley
  • Rats
  • Prenatal Exposure Delayed Effects
  • Pregnancy
  • Neurotoxins
  • Neurology & Neurosurgery
  • Neuroglia
  • Male
  • Insecticides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Garcia, S. J., Seidler, F. J., Qiao, D., & Slotkin, T. A. (2002). Chlorpyrifos targets developing glia: effects on glial fibrillary acidic protein. Brain Res Dev Brain Res, 133(2), 151–161. https://doi.org/10.1016/s0165-3806(02)00283-3
Garcia, Stephanie J., Frederic J. Seidler, Dan Qiao, and Theodore A. Slotkin. “Chlorpyrifos targets developing glia: effects on glial fibrillary acidic protein.Brain Res Dev Brain Res 133, no. 2 (February 28, 2002): 151–61. https://doi.org/10.1016/s0165-3806(02)00283-3.
Garcia SJ, Seidler FJ, Qiao D, Slotkin TA. Chlorpyrifos targets developing glia: effects on glial fibrillary acidic protein. Brain Res Dev Brain Res. 2002 Feb 28;133(2):151–61.
Garcia, Stephanie J., et al. “Chlorpyrifos targets developing glia: effects on glial fibrillary acidic protein.Brain Res Dev Brain Res, vol. 133, no. 2, Feb. 2002, pp. 151–61. Pubmed, doi:10.1016/s0165-3806(02)00283-3.
Garcia SJ, Seidler FJ, Qiao D, Slotkin TA. Chlorpyrifos targets developing glia: effects on glial fibrillary acidic protein. Brain Res Dev Brain Res. 2002 Feb 28;133(2):151–161.
Journal cover image

Published In

Brain Res Dev Brain Res

DOI

ISSN

0165-3806

Publication Date

February 28, 2002

Volume

133

Issue

2

Start / End Page

151 / 161

Location

Netherlands

Related Subject Headings

  • Sex Factors
  • Rats, Sprague-Dawley
  • Rats
  • Prenatal Exposure Delayed Effects
  • Pregnancy
  • Neurotoxins
  • Neurology & Neurosurgery
  • Neuroglia
  • Male
  • Insecticides