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Prenatal cocaine and cell development in rat brain regions: effects on ornithine decarboxylase and macromolecules.

Publication ,  Journal Article
Seidler, FJ; Slotkin, TA
Published in: Brain Res Bull
1993

Prenatal cocaine exposure has been shown to cause neurobehavioral abnormalities. To determine whether effects on basic patterns of cell development underlie these functional deficits, we examined the aftermath of acute and chronic cocaine exposure on ontogenetic patterns of ornithine decarboxylase (ODC), a key regulator of cell replication/differentiation, DNA synthesis as monitored by [3H]thymidine incorporation, and markers of cell number (DNA content) and cell size (protein/DNA ratio). Administration of 30 mg/kg SC of cocaine to pregnant rats on gestational day 20 resulted in acute increases of ODC throughout the brain. When the same dose of cocaine was given daily from gestational days 8 through 20, ODC elevations persisted into the neonatal period but disappeared by the middle of the first postnatal week. Although this treatment regimen retarded maternal weight gain, there was little or no effect on pup body or brain region weights. Similarly, minor changes in DNA synthesis were seen in two brain regions (forebrain, cerebellum), but DNA content was largely unaffected. Postnatal cell growth was significantly reduced in the forebrain, as evidenced by deficits in protein/DNA but, again, the magnitude of effect was quite small. Raising the daily dose of cocaine to 100 mg/kg resulted in significant maternal mortality and fetal resorptions in surviving dams. Shortening the treatment regimen to a 3-day period (gestational days 18 through 20) eliminated the effects on maternal weight gain and on postnatal pup brain region ODC.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Brain Res Bull

DOI

ISSN

0361-9230

Publication Date

1993

Volume

30

Issue

1-2

Start / End Page

91 / 99

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Proteins
  • Prosencephalon
  • Prenatal Exposure Delayed Effects
  • Pregnancy
  • Ornithine Decarboxylase
  • Organ Size
  • Neurology & Neurosurgery
  • Mesencephalon
 

Citation

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Seidler, F. J., & Slotkin, T. A. (1993). Prenatal cocaine and cell development in rat brain regions: effects on ornithine decarboxylase and macromolecules. Brain Res Bull, 30(1–2), 91–99. https://doi.org/10.1016/0361-9230(93)90043-b
Seidler, F. J., and T. A. Slotkin. “Prenatal cocaine and cell development in rat brain regions: effects on ornithine decarboxylase and macromolecules.Brain Res Bull 30, no. 1–2 (1993): 91–99. https://doi.org/10.1016/0361-9230(93)90043-b.
Seidler, F. J., and T. A. Slotkin. “Prenatal cocaine and cell development in rat brain regions: effects on ornithine decarboxylase and macromolecules.Brain Res Bull, vol. 30, no. 1–2, 1993, pp. 91–99. Pubmed, doi:10.1016/0361-9230(93)90043-b.
Journal cover image

Published In

Brain Res Bull

DOI

ISSN

0361-9230

Publication Date

1993

Volume

30

Issue

1-2

Start / End Page

91 / 99

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Proteins
  • Prosencephalon
  • Prenatal Exposure Delayed Effects
  • Pregnancy
  • Ornithine Decarboxylase
  • Organ Size
  • Neurology & Neurosurgery
  • Mesencephalon