Skip to main content
Journal cover image

Macromolecular levels, DNA synthesis and ornithine decarboxylase activity in leg muscles from 6-mercaptopurine-treated rats.

Publication ,  Journal Article
Friedman, L; Gaines, DW; Alleva, FR; Seidler, FJ; Flynn, TJ; Slotkin, TA; Balazs, T
Published in: Toxicol Ind Health
September 1986

Sprague-Dawley male and female rats were treated with 6-mercaptopurine (6-MP) (2 mg/kg sc) daily from 2 to 22 days of age and killed at 7, 15, 27 and 64 days of age. At 7 and 27 days of age rats were injected with 3H thymidine for measurement of DNA synthesis. Fore- and hindlimb muscles were removed and analyzed for ornithine decarboxylase (ODC) activity (all ages), DNA radioactivity (7 and 27 days), DNA level (27 and 64 days) and RNA level (64 days). As expected, ODC activity and DNA synthesis were higher in muscles of 7-day-old rats than in muscles of the older rats studied. A consistently lower ODC activity was seen in 6-MP-treated vs. control rats for 5-25 days after start of treatment, but the effect was essentially the same for the hindlimb and forelimb muscles. During the 7-27-day time course ODC activity was higher in hindlimb than forelimb muscles. By 27 days of age DNA synthesis was also higher in the hindlimb muscles. DNA synthesis was decreased after 5 days of treatment relative to that of control rats, to an approximately equal extent in forelimb and hindlimb muscles. Five days after the last treatment a trend was seen for slower recovery from inhibition of DNA synthesis in hindlimb muscles, particularly in male rats. DNA levels were reduced in treated rats relative to those in control rats 5 days after the last treatment to approximately the same degree in forelimb and hindlimb muscles. Forty-two days after the last treatment a trend toward increased activity of ODC and increased DNA and RNA levels was seen in muscles of treated rats, probably reflective of recovery processes. These early biochemical effects of 6-MP, which were seen to about the same extent in the forelimb and hindlimb muscles cannot explain by themselves the delayed hindlimb fat atrophy resulting from 6-MP treatment of neonatal rats.

Duke Scholars

Published In

Toxicol Ind Health

DOI

ISSN

0748-2337

Publication Date

September 1986

Volume

2

Issue

2

Start / End Page

57 / 68

Location

England

Related Subject Headings

  • Toxicology
  • Rats, Inbred Strains
  • Rats
  • RNA
  • Ornithine Decarboxylase
  • Muscles
  • Muscle Proteins
  • Mercaptopurine
  • Male
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Friedman, L., Gaines, D. W., Alleva, F. R., Seidler, F. J., Flynn, T. J., Slotkin, T. A., & Balazs, T. (1986). Macromolecular levels, DNA synthesis and ornithine decarboxylase activity in leg muscles from 6-mercaptopurine-treated rats. Toxicol Ind Health, 2(2), 57–68. https://doi.org/10.1177/074823378600200207
Friedman, L., D. W. Gaines, F. R. Alleva, F. J. Seidler, T. J. Flynn, T. A. Slotkin, and T. Balazs. “Macromolecular levels, DNA synthesis and ornithine decarboxylase activity in leg muscles from 6-mercaptopurine-treated rats.Toxicol Ind Health 2, no. 2 (September 1986): 57–68. https://doi.org/10.1177/074823378600200207.
Friedman L, Gaines DW, Alleva FR, Seidler FJ, Flynn TJ, Slotkin TA, et al. Macromolecular levels, DNA synthesis and ornithine decarboxylase activity in leg muscles from 6-mercaptopurine-treated rats. Toxicol Ind Health. 1986 Sep;2(2):57–68.
Friedman, L., et al. “Macromolecular levels, DNA synthesis and ornithine decarboxylase activity in leg muscles from 6-mercaptopurine-treated rats.Toxicol Ind Health, vol. 2, no. 2, Sept. 1986, pp. 57–68. Pubmed, doi:10.1177/074823378600200207.
Friedman L, Gaines DW, Alleva FR, Seidler FJ, Flynn TJ, Slotkin TA, Balazs T. Macromolecular levels, DNA synthesis and ornithine decarboxylase activity in leg muscles from 6-mercaptopurine-treated rats. Toxicol Ind Health. 1986 Sep;2(2):57–68.
Journal cover image

Published In

Toxicol Ind Health

DOI

ISSN

0748-2337

Publication Date

September 1986

Volume

2

Issue

2

Start / End Page

57 / 68

Location

England

Related Subject Headings

  • Toxicology
  • Rats, Inbred Strains
  • Rats
  • RNA
  • Ornithine Decarboxylase
  • Muscles
  • Muscle Proteins
  • Mercaptopurine
  • Male
  • Female