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Ontogeny of (--)-[3H]norepinephrine uptake properties of synaptic storage vesicles of rat brain.

Publication ,  Journal Article
Kirksey, DF; Seidler, FJ; Slotkin, TA
Published in: Brain Res
July 14, 1978

The ontogeny of[3H]norepinephrine uptake mechanisms has been examined in synaptosomes and storage vesicles isolated from rat whole brain. The [3H]norepinephrine accumulated by synaptosomes was low in neonates, but reached adult levels by 15 days of age. In contrast, development of[3H]norepinephrine uptake into isolated rat brain storage vesicles was not complete until 38 days of age. Kinetic analysis of the developing vesicular uptake mechanism revealed no change in Km, while maximal uptake increased progressively from birth to maturity. Storage vesicles from immature and adult rats exhibited similar energy requirements for uptake as determined by their dependence on ATP-Mg2+ concentration; furthermore, the degree of inhibition of [3H]norepinephrine uptake by other amines was the same in both vesicle preparations. Thus, storage vesicles isolated from adult and developing rats display an in vitro[3H]norepinephrine uptake mechanism with properties that are kinetically and pharmacologically similar. The results suggest that, while the number of storage vesicles in the central nervous system increases during development, those vesicles that are present possess a fully functional amine uptake system.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

July 14, 1978

Volume

150

Issue

2

Start / End Page

367 / 375

Location

Netherlands

Related Subject Headings

  • Synaptosomes
  • Synaptic Vesicles
  • Rats
  • Organ Size
  • Norepinephrine
  • Neurology & Neurosurgery
  • Kinetics
  • In Vitro Techniques
  • Cell Count
  • Brain
 

Citation

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Kirksey, D. F., Seidler, F. J., & Slotkin, T. A. (1978). Ontogeny of (--)-[3H]norepinephrine uptake properties of synaptic storage vesicles of rat brain. Brain Res, 150(2), 367–375. https://doi.org/10.1016/0006-8993(78)90287-1
Kirksey, D. F., F. J. Seidler, and T. A. Slotkin. “Ontogeny of (--)-[3H]norepinephrine uptake properties of synaptic storage vesicles of rat brain.Brain Res 150, no. 2 (July 14, 1978): 367–75. https://doi.org/10.1016/0006-8993(78)90287-1.
Kirksey DF, Seidler FJ, Slotkin TA. Ontogeny of (--)-[3H]norepinephrine uptake properties of synaptic storage vesicles of rat brain. Brain Res. 1978 Jul 14;150(2):367–75.
Kirksey, D. F., et al. “Ontogeny of (--)-[3H]norepinephrine uptake properties of synaptic storage vesicles of rat brain.Brain Res, vol. 150, no. 2, July 1978, pp. 367–75. Pubmed, doi:10.1016/0006-8993(78)90287-1.
Kirksey DF, Seidler FJ, Slotkin TA. Ontogeny of (--)-[3H]norepinephrine uptake properties of synaptic storage vesicles of rat brain. Brain Res. 1978 Jul 14;150(2):367–375.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

July 14, 1978

Volume

150

Issue

2

Start / End Page

367 / 375

Location

Netherlands

Related Subject Headings

  • Synaptosomes
  • Synaptic Vesicles
  • Rats
  • Organ Size
  • Norepinephrine
  • Neurology & Neurosurgery
  • Kinetics
  • In Vitro Techniques
  • Cell Count
  • Brain