Skip to main content
Journal cover image

Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells.

Publication ,  Journal Article
Abreu-Villaça, Y; Seidler, FJ; Qiao, D; Slotkin, TA
Published in: Brain Res Dev Brain Res
February 8, 2005

Although nicotine is a developmental neurotoxicant, it also can exert neuroprotective effects. In the current study, we used PC12 cells to determine the developmental phases in which these disparate actions are expressed and to compare the concentrations required for each. In undifferentiated cells, 1 or 10 microM nicotine had little or no effect on cell number (assessed by measuring DNA) but exerted positive trophic actions, characterized by transient enhancement of cell growth (increased total protein/DNA ratio) and persistent enhancement of cell viability (decreased proportions of cells stained with trypan blue). When differentiation was initiated with nerve growth factor, nicotine elicited a different spectrum of actions, with decreases in cell number, impaired neuritic outgrowth (reduced ratio of membrane/total protein) and weakened viability. In either undifferentiated or differentiating cells, nicotine increased lipid peroxidation (determined as thiobarbituric acid reactive species), providing evidence for oxidative damage. Our results indicate that nicotine exerts positive trophic effects primarily on undifferentiated cells, whereas with differentiation the effects undergo a transition to neurotoxicity. These findings support the view that the neurodevelopmental actions of nicotine depend not only upon the magnitude and duration of the exposure, but most importantly on the developmental stage (e.g., differentiation state) in which exposure occurs.

Duke Scholars

Published In

Brain Res Dev Brain Res

DOI

ISSN

0165-3806

Publication Date

February 8, 2005

Volume

154

Issue

2

Start / End Page

239 / 246

Location

Netherlands

Related Subject Headings

  • Thiobarbituric Acid Reactive Substances
  • Staining and Labeling
  • Rats
  • PC12 Cells
  • Nicotine
  • Neurotoxins
  • Neurology & Neurosurgery
  • Nerve Growth Factor
  • Drug Interactions
  • Dose-Response Relationship, Drug
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Abreu-Villaça, Y., Seidler, F. J., Qiao, D., & Slotkin, T. A. (2005). Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells. Brain Res Dev Brain Res, 154(2), 239–246. https://doi.org/10.1016/j.devbrainres.2004.10.018
Abreu-Villaça, Yael, Frederic J. Seidler, Dan Qiao, and Theodore A. Slotkin. “Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells.Brain Res Dev Brain Res 154, no. 2 (February 8, 2005): 239–46. https://doi.org/10.1016/j.devbrainres.2004.10.018.
Abreu-Villaça Y, Seidler FJ, Qiao D, Slotkin TA. Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells. Brain Res Dev Brain Res. 2005 Feb 8;154(2):239–46.
Abreu-Villaça, Yael, et al. “Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells.Brain Res Dev Brain Res, vol. 154, no. 2, Feb. 2005, pp. 239–46. Pubmed, doi:10.1016/j.devbrainres.2004.10.018.
Abreu-Villaça Y, Seidler FJ, Qiao D, Slotkin TA. Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells. Brain Res Dev Brain Res. 2005 Feb 8;154(2):239–246.
Journal cover image

Published In

Brain Res Dev Brain Res

DOI

ISSN

0165-3806

Publication Date

February 8, 2005

Volume

154

Issue

2

Start / End Page

239 / 246

Location

Netherlands

Related Subject Headings

  • Thiobarbituric Acid Reactive Substances
  • Staining and Labeling
  • Rats
  • PC12 Cells
  • Nicotine
  • Neurotoxins
  • Neurology & Neurosurgery
  • Nerve Growth Factor
  • Drug Interactions
  • Dose-Response Relationship, Drug