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Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase.

Publication ,  Journal Article
Bowes, C; Li, T; Frankel, WN; Danciger, M; Coffin, JM; Applebury, ML; Farber, DB
Published in: Proc Natl Acad Sci U S A
April 1, 1993
Published version (DOI) Link to item

Retinal degeneration in the rd mouse is inherited as an autosomal recessive trait and is caused by a defect in the gene encoding the beta subunit of cGMP phosphodiesterase. Recently, a close genetic association of the rd gene with an endogenous xenotropic murine leukemia virus (Xmv-28) was established by linkage analysis using recombinant inbred strains of mice. In this study, genomic DNA mapping and sequence analyses clarify the position of the proviral sequences in relation to the rd gene. We find that the Xmv-28 provirus is integrated into intron I of the rd gene 1511 bp downstream of the exon-intron boundary. The transcriptional orientation of the provirus is opposite to that of the gene for the beta subunit of cGMP phosphodiesterase. Reverse transcription-PCR demonstrates that the integrated Xmv-28 sequences are transcribed in the retina. The provirus is present in every strain of rd mouse tested.

Duke Scholars

Catherine Bowes Rickman
Author Catherine Bowes Rickman Ophthalmology, Vitreoretinal Diseases & Surgery

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.90.7.2955

ISSN

0027-8424

Publication Date

April 1, 1993

Volume

90

Issue

7

Start / End Page

2955 / 2959

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Retroviridae
  • Restriction Mapping
  • Polymerase Chain Reaction
  • Phosphoric Diester Hydrolases
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
 

Citation

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MLA
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Bowes, C., Li, T., Frankel, W. N., Danciger, M., Coffin, J. M., Applebury, M. L., & Farber, D. B. (1993). Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase. Proc Natl Acad Sci U S A, 90(7), 2955–2959. https://doi.org/10.1073/pnas.90.7.2955
Bowes, C., T. Li, W. N. Frankel, M. Danciger, J. M. Coffin, M. L. Applebury, and D. B. Farber. “Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase.” Proc Natl Acad Sci U S A 90, no. 7 (April 1, 1993): 2955–59. https://doi.org/10.1073/pnas.90.7.2955.
Bowes C, Li T, Frankel WN, Danciger M, Coffin JM, Applebury ML, et al. Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2955–9.
Bowes, C., et al. “Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase.” Proc Natl Acad Sci U S A, vol. 90, no. 7, Apr. 1993, pp. 2955–59. Pubmed, doi:10.1073/pnas.90.7.2955.
Bowes C, Li T, Frankel WN, Danciger M, Coffin JM, Applebury ML, Farber DB. Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2955–2959.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.90.7.2955

ISSN

0027-8424

Publication Date

April 1, 1993

Volume

90

Issue

7

Start / End Page

2955 / 2959

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Retroviridae
  • Restriction Mapping
  • Polymerase Chain Reaction
  • Phosphoric Diester Hydrolases
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice