
Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes.
The effects of various barbiturates and picrotoxin in modifying the efflux of chloride (36Cl-) was studied in a novel subcellular preparation from rat cerebral cortex, the 'synaptoneurosome'. Dilution of synaptoneurosomes pre-loaded with 36Cl- resulted in rapid efflux of 36Cl- that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in 36Cl- efflux which was not observed with the pharmacologically-inactive barbiturate, barbital. The effect of barbiturates in enhancing 36Cl- efflux was also stereospecific [(-)-DMBB greater than (+)-DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited 36Cl- efflux. These data demonstrate pharmacologically relevant Cl- transport for the first time in a subcellular brain preparation.
Duke Scholars
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Synaptosomes
- Structure-Activity Relationship
- Rats, Inbred Strains
- Rats
- Picrotoxin
- Neurons
- Male
- Kinetics
- Chlorides
- Cerebral Cortex
Citation

Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Synaptosomes
- Structure-Activity Relationship
- Rats, Inbred Strains
- Rats
- Picrotoxin
- Neurons
- Male
- Kinetics
- Chlorides
- Cerebral Cortex