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Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I.

Publication ,  Journal Article
Cegielska, A; Virshup, DM
Published in: Mol Cell Biol
February 1993

The initiation of simian virus 40 (SV40) DNA replication is regulated by the phosphorylation state of the viral initiator protein, large T antigen. We describe the purification from HeLa cell nuclei of a 35-kDa serine/threonine protein kinase that phosphorylates T antigen at sites that are phosphorylated in vivo and thereby inhibits its ability to initiate SV40 DNA replication. The inhibition of both origin unwinding and DNA replication by the kinase is reversed by protein phosphatase 2A. As determined by molecular weight, substrate specificity, autophosphorylation, immunoreactivity, and limited sequence analysis, this kinase appears to be identical to casein kinase I, a ubiquitous serine/threonine protein kinase that is closely related to a yeast kinase involved in DNA metabolism. The HeLa cell phosphorylation cycle that controls the initiation of SV40 DNA replication may also play a role in cellular DNA metabolism.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

February 1993

Volume

13

Issue

2

Start / End Page

1202 / 1211

Location

United States

Related Subject Headings

  • Temperature
  • Simian virus 40
  • Protein Phosphatase 2
  • Protein Kinases
  • Protein Kinase Inhibitors
  • Phosphorylation
  • Phosphoprotein Phosphatases
  • Molecular Sequence Data
  • Humans
  • Hela Cells
 

Citation

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Cegielska, A., & Virshup, D. M. (1993). Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I. Mol Cell Biol, 13(2), 1202–1211. https://doi.org/10.1128/mcb.13.2.1202-1211.1993
Cegielska, A., and D. M. Virshup. “Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I.Mol Cell Biol 13, no. 2 (February 1993): 1202–11. https://doi.org/10.1128/mcb.13.2.1202-1211.1993.
Cegielska A, Virshup DM. Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I. Mol Cell Biol. 1993 Feb;13(2):1202–11.
Cegielska, A., and D. M. Virshup. “Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I.Mol Cell Biol, vol. 13, no. 2, Feb. 1993, pp. 1202–11. Pubmed, doi:10.1128/mcb.13.2.1202-1211.1993.
Cegielska A, Virshup DM. Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I. Mol Cell Biol. 1993 Feb;13(2):1202–1211.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

February 1993

Volume

13

Issue

2

Start / End Page

1202 / 1211

Location

United States

Related Subject Headings

  • Temperature
  • Simian virus 40
  • Protein Phosphatase 2
  • Protein Kinases
  • Protein Kinase Inhibitors
  • Phosphorylation
  • Phosphoprotein Phosphatases
  • Molecular Sequence Data
  • Humans
  • Hela Cells