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The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.

Publication ,  Journal Article
McCright, B; Rivers, AM; Audlin, S; Virshup, DM
Published in: J Biol Chem
September 6, 1996

Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kDa catalytic C subunit, a 65-kDa structural A subunit, and a variable regulatory B subunit. The B subunits determine the substrate specificity of the enzyme. There have been three families of cellular B subunits identified to date: B55, B56 (B'), and PR72/130. We have now cloned five genes encoding human B56 isoforms. Polypeptides encoded by all but one splice variant (B56gamma1) are phosphoproteins, as shown by mobility shift after treatment with alkaline phosphatase and metabolic labeling with [32P]phosphate. All labeled isoforms contain solely phosphoserine. Indirect immunofluorescence microscopy demonstrates distinct patterns of intracellular targeting by different B56 isoforms. Specifically, B56alpha, B56beta, and B56epsilon complexed with the protein phosphatase 2A A and C subunits localize to the cytoplasm, whereas B56delta, B56gamma1, and B56gamma3 are concentrated in the nucleus. Two isoforms (B56beta and B56delta) are highly expressed in adult brain; here we show that mRNA for these isoforms increases severalfold when neuroblastoma cell lines are induced to differentiate by retinoic acid treatment. These studies demonstrate an increasing diversity of regulatory mechanisms to control the activity of this key intracellular protein phosphatase and suggest distinct functions for isoforms targeted to different intracellular locations.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

September 6, 1996

Volume

271

Issue

36

Start / End Page

22081 / 22089

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tretinoin
  • Structure-Activity Relationship
  • Software
  • Sequence Homology, Nucleic Acid
  • Protein Phosphatase 2
  • Protein Conformation
  • Phosphorylation
  • Phosphoproteins
  • Phosphoprotein Phosphatases
 

Citation

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McCright, B., Rivers, A. M., Audlin, S., & Virshup, D. M. (1996). The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm. J Biol Chem, 271(36), 22081–22089. https://doi.org/10.1074/jbc.271.36.22081
McCright, B., A. M. Rivers, S. Audlin, and D. M. Virshup. “The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.J Biol Chem 271, no. 36 (September 6, 1996): 22081–89. https://doi.org/10.1074/jbc.271.36.22081.
McCright, B., et al. “The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.J Biol Chem, vol. 271, no. 36, Sept. 1996, pp. 22081–89. Pubmed, doi:10.1074/jbc.271.36.22081.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

September 6, 1996

Volume

271

Issue

36

Start / End Page

22081 / 22089

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tretinoin
  • Structure-Activity Relationship
  • Software
  • Sequence Homology, Nucleic Acid
  • Protein Phosphatase 2
  • Protein Conformation
  • Phosphorylation
  • Phosphoproteins
  • Phosphoprotein Phosphatases