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In vitro versus in vivo correlations of chemosensitivity of human medulloblastoma.

Publication ,  Journal Article
Friedman, HS; Schold, SC; Muhlbaier, LH; Bjornsson, TD; Bigner, DD
Published in: Cancer Res
November 1984

An in vitro clonogenic assay was used to test the chemosensitivity of the human medulloblastoma cell line TE-671. Dose-response relationships for reduction in colony formation were generated for cyclophosphamide, vincristine, Adriamycin, 1,3-bis(2-chloroethyl)-1-nitrosourea (NSC 409962), and 1,4-cyclohexadiene-1,4-dicarbamic acid, 2,5-bis(1-aziridinyl)-3,6-dioxo-diethylester (NSC 182986); and the in vitro drug dose at which there is a 75% reduction in the number of colonies in comparison to controls (ID75S) were derived from these data. Methotrexate produced no colony reduction at any dose tested up to 1000 micrograms/ml. The in vitro results were compared to growth delays in s.c. TE-671 xenografts in athymic mice treated with the same agents. Agents with an ID75 less than assumed in vivo plasma drug concentrations were all active in vivo, whereas two of the three agents with an ID75 greater than assumed in vivo plasma drug concentrations demonstrated no in vivo activity. These results suggest that for these agents, the relationship between the ID75 of the drug and its in vivo concentration allows in vitro clonogenic assay results to agree with in vivo growth delay responses.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

November 1984

Volume

44

Issue

11

Start / End Page

5145 / 5149

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Humans
  • Drug Evaluation, Preclinical
  • Clone Cells
  • Cerebellar Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Friedman, H. S., Schold, S. C., Muhlbaier, L. H., Bjornsson, T. D., & Bigner, D. D. (1984). In vitro versus in vivo correlations of chemosensitivity of human medulloblastoma. Cancer Res, 44(11), 5145–5149.
Friedman, H. S., S. C. Schold, L. H. Muhlbaier, T. D. Bjornsson, and D. D. Bigner. “In vitro versus in vivo correlations of chemosensitivity of human medulloblastoma.Cancer Res 44, no. 11 (November 1984): 5145–49.
Friedman HS, Schold SC, Muhlbaier LH, Bjornsson TD, Bigner DD. In vitro versus in vivo correlations of chemosensitivity of human medulloblastoma. Cancer Res. 1984 Nov;44(11):5145–9.
Friedman, H. S., et al. “In vitro versus in vivo correlations of chemosensitivity of human medulloblastoma.Cancer Res, vol. 44, no. 11, Nov. 1984, pp. 5145–49.
Friedman HS, Schold SC, Muhlbaier LH, Bjornsson TD, Bigner DD. In vitro versus in vivo correlations of chemosensitivity of human medulloblastoma. Cancer Res. 1984 Nov;44(11):5145–5149.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

November 1984

Volume

44

Issue

11

Start / End Page

5145 / 5149

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Humans
  • Drug Evaluation, Preclinical
  • Clone Cells
  • Cerebellar Neoplasms