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Specificity and regulation of a synaptic vesicle docking complex.

Publication ,  Journal Article
Pevsner, J; Hsu, SC; Braun, JE; Calakos, N; Ting, AE; Bennett, MK; Scheller, RH
Published in: Neuron
August 1994

Synaptic vesicles are proposed to dock at the presynaptic plasma membrane through the interaction of two integral membrane proteins of synaptic vesicles, VAMP and synaptotagmin, and two plasma membrane proteins, syntaxin and SNAP-25. We have characterized the binding properties of these proteins and observed SNAP-25 potentiation of VAMP 2 binding to syntaxins 1a and 4 but not syntaxins 2 or 3. n-sec1, a neuron-specific syntaxin-binding protein, bound syntaxin with nanomolar affinity, forming a complex that is distinct from the previously identified 7S and 20S syntaxin-containing complexes. This suggests that syntaxin exists in at least three states: bound to n-sec1, in a 7S particle, and in a 20S particle. Recombinant n-sec1 inhibited VAMP or SNAP-25 binding to syntaxin. We propose that the specific associations of VAMP, SNAP-25, and syntaxin mediate vesicle docking and that a syntaxin/n-sec1 complex precedes and/or regulates formation of these complexes.

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Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

August 1994

Volume

13

Issue

2

Start / End Page

353 / 361

Location

United States

Related Subject Headings

  • Vesicular Transport Proteins
  • Synaptosomal-Associated Protein 25
  • Synaptic Vesicles
  • Recombinant Fusion Proteins
  • Rats
  • R-SNARE Proteins
  • Protein Binding
  • Peptides
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
 

Citation

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ICMJE
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Pevsner, J., Hsu, S. C., Braun, J. E., Calakos, N., Ting, A. E., Bennett, M. K., & Scheller, R. H. (1994). Specificity and regulation of a synaptic vesicle docking complex. Neuron, 13(2), 353–361. https://doi.org/10.1016/0896-6273(94)90352-2
Pevsner, J., S. C. Hsu, J. E. Braun, N. Calakos, A. E. Ting, M. K. Bennett, and R. H. Scheller. “Specificity and regulation of a synaptic vesicle docking complex.Neuron 13, no. 2 (August 1994): 353–61. https://doi.org/10.1016/0896-6273(94)90352-2.
Pevsner J, Hsu SC, Braun JE, Calakos N, Ting AE, Bennett MK, et al. Specificity and regulation of a synaptic vesicle docking complex. Neuron. 1994 Aug;13(2):353–61.
Pevsner, J., et al. “Specificity and regulation of a synaptic vesicle docking complex.Neuron, vol. 13, no. 2, Aug. 1994, pp. 353–61. Pubmed, doi:10.1016/0896-6273(94)90352-2.
Pevsner J, Hsu SC, Braun JE, Calakos N, Ting AE, Bennett MK, Scheller RH. Specificity and regulation of a synaptic vesicle docking complex. Neuron. 1994 Aug;13(2):353–361.
Journal cover image

Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

August 1994

Volume

13

Issue

2

Start / End Page

353 / 361

Location

United States

Related Subject Headings

  • Vesicular Transport Proteins
  • Synaptosomal-Associated Protein 25
  • Synaptic Vesicles
  • Recombinant Fusion Proteins
  • Rats
  • R-SNARE Proteins
  • Protein Binding
  • Peptides
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins