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Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture.

Publication ,  Journal Article
Wang, JY; Baer, AE; Kraus, VB; Setton, LA
Published in: Spine (Phila Pa 1976)
August 15, 2001

STUDY DESIGN: The mRNA levels of aggrecan and collagen were quantified in intervertebral disc cells cultured under three conditions: primary alginate culture, monolayer culture, and re-encapsulation in alginate after monolayer culture. OBJECTIVES: To compare the phenotype of intervertebral disc cells under different culture conditions and to investigate the reversibility of cell phenotype after re-encapsulation in alginate after monolayer culture. SUMMARY OF BACKGROUND DATA: The intervertebral disc contains heterogeneous populations of cells that vary with anatomic region. These cells possess significant differences in phenotype that can be preserved in vitro, although the effect of culture conditions on the phenotype of these cells is poorly understood. METHODS: The intervertebral disc cells of 4-5-month-old pigs were isolated enzymatically from three anatomic zones: anulus fibrosus (AF), transition zone (TZ), and nucleus pulposus (NP). Gene expression levels of aggrecan and collagen Types I and II were measured using a quantitative reverse transcriptase--polymerase chain reaction. RESULTS: Gene expression levels of anulus fibrosus and transition zone cells were shifted in monolayer compared with alginate, although the shift was partially reversed when re-encapsulated in alginate. However, NP cells appeared to be insensitive to culture conditions. Furthermore, characteristic patterns of gene expression among AF, TZ, and NP cells in primary alginate culture did not exist in monolayer culture, but they were also observed after re-encapsulation in alginate. CONCLUSION: The findings of this study suggest that anulus fibrosus and transition zone cells undergo a reversible shift in phenotype when cultured in monolayer compared with alginate. These differences suggest that the culture system exerts a strong influence on cell phenotype and may play a role in the response of these cells to biophysical and biochemical stimuli in vitro.

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Published In

Spine (Phila Pa 1976)

DOI

ISSN

0362-2436

Publication Date

August 15, 2001

Volume

26

Issue

16

Start / End Page

1747 / 1751

Location

United States

Related Subject Headings

  • Swine
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Proteoglycans
  • Phenotype
  • Orthopedics
  • Lumbar Vertebrae
  • Lectins, C-Type
  • Intervertebral Disc
  • Hexuronic Acids
 

Citation

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Wang, J. Y., Baer, A. E., Kraus, V. B., & Setton, L. A. (2001). Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. Spine (Phila Pa 1976), 26(16), 1747–1751. https://doi.org/10.1097/00007632-200108150-00003
Wang, J. Y., A. E. Baer, V. B. Kraus, and L. A. Setton. “Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture.Spine (Phila Pa 1976) 26, no. 16 (August 15, 2001): 1747–51. https://doi.org/10.1097/00007632-200108150-00003.
Wang JY, Baer AE, Kraus VB, Setton LA. Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. Spine (Phila Pa 1976). 2001 Aug 15;26(16):1747–51.
Wang, J. Y., et al. “Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture.Spine (Phila Pa 1976), vol. 26, no. 16, Aug. 2001, pp. 1747–51. Pubmed, doi:10.1097/00007632-200108150-00003.
Wang JY, Baer AE, Kraus VB, Setton LA. Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. Spine (Phila Pa 1976). 2001 Aug 15;26(16):1747–1751.

Published In

Spine (Phila Pa 1976)

DOI

ISSN

0362-2436

Publication Date

August 15, 2001

Volume

26

Issue

16

Start / End Page

1747 / 1751

Location

United States

Related Subject Headings

  • Swine
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Proteoglycans
  • Phenotype
  • Orthopedics
  • Lumbar Vertebrae
  • Lectins, C-Type
  • Intervertebral Disc
  • Hexuronic Acids