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Single protein misfolding events captured by atomic force microscopy.

Publication ,  Journal Article
Oberhauser, AF; Marszalek, PE; Carrion-Vazquez, M; Fernandez, JM
Published in: Nature structural biology
November 1999

Using single protein atomic force microscopy (AFM) techniques we demonstrate that after repeated mechanical extension/relaxation cycles, tandem modular proteins can misfold into a structure formed by two neighboring modules. The misfolding is fully reversible and alters the mechanical topology of the modules while it is about as stable as the original fold. Our results show that modular proteins can assume a novel misfolded state and demonstrate that AFM is able to capture, in real time, rare misfolding events at the level of a single protein.

Duke Scholars

Published In

Nature structural biology

DOI

ISSN

1072-8368

Publication Date

November 1999

Volume

6

Issue

11

Start / End Page

1025 / 1028

Related Subject Headings

  • Repetitive Sequences, Amino Acid
  • Protein Structure, Tertiary
  • Protein Structure, Secondary
  • Protein Renaturation
  • Protein Kinases
  • Protein Folding
  • Protein Engineering
  • Protein Denaturation
  • Polyproteins
  • Myocardium
 

Citation

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Chicago
ICMJE
MLA
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Oberhauser, A. F., Marszalek, P. E., Carrion-Vazquez, M., & Fernandez, J. M. (1999). Single protein misfolding events captured by atomic force microscopy. Nature Structural Biology, 6(11), 1025–1028. https://doi.org/10.1038/14907
Oberhauser, A. F., P. E. Marszalek, M. Carrion-Vazquez, and J. M. Fernandez. “Single protein misfolding events captured by atomic force microscopy.Nature Structural Biology 6, no. 11 (November 1999): 1025–28. https://doi.org/10.1038/14907.
Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Single protein misfolding events captured by atomic force microscopy. Nature structural biology. 1999 Nov;6(11):1025–8.
Oberhauser, A. F., et al. “Single protein misfolding events captured by atomic force microscopy.Nature Structural Biology, vol. 6, no. 11, Nov. 1999, pp. 1025–28. Epmc, doi:10.1038/14907.
Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Single protein misfolding events captured by atomic force microscopy. Nature structural biology. 1999 Nov;6(11):1025–1028.

Published In

Nature structural biology

DOI

ISSN

1072-8368

Publication Date

November 1999

Volume

6

Issue

11

Start / End Page

1025 / 1028

Related Subject Headings

  • Repetitive Sequences, Amino Acid
  • Protein Structure, Tertiary
  • Protein Structure, Secondary
  • Protein Renaturation
  • Protein Kinases
  • Protein Folding
  • Protein Engineering
  • Protein Denaturation
  • Polyproteins
  • Myocardium