Single protein misfolding events captured by atomic force microscopy.
Publication
, Journal Article
Oberhauser, AF; Marszalek, PE; Carrion-Vazquez, M; Fernandez, JM
Published in: Nature structural biology
November 1999
Using single protein atomic force microscopy (AFM) techniques we demonstrate that after repeated mechanical extension/relaxation cycles, tandem modular proteins can misfold into a structure formed by two neighboring modules. The misfolding is fully reversible and alters the mechanical topology of the modules while it is about as stable as the original fold. Our results show that modular proteins can assume a novel misfolded state and demonstrate that AFM is able to capture, in real time, rare misfolding events at the level of a single protein.
Duke Scholars
Published In
Nature structural biology
DOI
ISSN
1072-8368
Publication Date
November 1999
Volume
6
Issue
11
Start / End Page
1025 / 1028
Related Subject Headings
- Repetitive Sequences, Amino Acid
- Protein Structure, Tertiary
- Protein Structure, Secondary
- Protein Renaturation
- Protein Kinases
- Protein Folding
- Protein Engineering
- Protein Denaturation
- Polyproteins
- Myocardium
Citation
APA
Chicago
ICMJE
MLA
NLM
Oberhauser, A. F., Marszalek, P. E., Carrion-Vazquez, M., & Fernandez, J. M. (1999). Single protein misfolding events captured by atomic force microscopy. Nature Structural Biology, 6(11), 1025–1028. https://doi.org/10.1038/14907
Oberhauser, A. F., P. E. Marszalek, M. Carrion-Vazquez, and J. M. Fernandez. “Single protein misfolding events captured by atomic force microscopy.” Nature Structural Biology 6, no. 11 (November 1999): 1025–28. https://doi.org/10.1038/14907.
Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Single protein misfolding events captured by atomic force microscopy. Nature structural biology. 1999 Nov;6(11):1025–8.
Oberhauser, A. F., et al. “Single protein misfolding events captured by atomic force microscopy.” Nature Structural Biology, vol. 6, no. 11, Nov. 1999, pp. 1025–28. Epmc, doi:10.1038/14907.
Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM. Single protein misfolding events captured by atomic force microscopy. Nature structural biology. 1999 Nov;6(11):1025–1028.
Published In
Nature structural biology
DOI
ISSN
1072-8368
Publication Date
November 1999
Volume
6
Issue
11
Start / End Page
1025 / 1028
Related Subject Headings
- Repetitive Sequences, Amino Acid
- Protein Structure, Tertiary
- Protein Structure, Secondary
- Protein Renaturation
- Protein Kinases
- Protein Folding
- Protein Engineering
- Protein Denaturation
- Polyproteins
- Myocardium