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Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo.

Publication ,  Journal Article
Oliveri, P; Walton, KD; Davidson, EH; McClay, DR
Published in: Development (Cambridge, England)
November 2006

The foxa gene is an integral component of the endoderm specification subcircuit of the endomesoderm gene regulatory network in the Strongylocentrotus purpuratus embryo. Its transcripts become confined to veg2, then veg1 endodermal territories, and, following gastrulation, throughout the gut. It is also expressed in the stomodeal ectoderm. gatae and otx genes provide input into the pregastrular regulatory system of foxa, and Foxa represses its own transcription, resulting in an oscillatory temporal expression profile. Here, we report three separate essential functions of the foxa gene: it represses mesodermal fate in the veg2 endomesoderm; it is required in postgastrular development for the expression of gut-specific genes; and it is necessary for stomodaeum formation. If its expression is reduced by a morpholino, more endomesoderm cells become pigment and other mesenchymal cell types, less gut is specified, and the larva has no mouth. Experiments in which blastomere transplantation is combined with foxa MASO treatment demonstrate that, in the normal endoderm, a crucial role of Foxa is to repress gcm expression in response to a Notch signal, and hence to repress mesodermal fate. Chimeric recombination experiments in which veg2, veg1 or ectoderm cells contained foxa MASO show which region of foxa expression controls each of the three functions. These experiments show that the foxa gene is a component of three distinct embryonic gene regulatory networks.

Duke Scholars

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

November 2006

Volume

133

Issue

21

Start / End Page

4173 / 4181

Related Subject Headings

  • Strongylocentrotus purpuratus
  • Signal Transduction
  • Recombinant Proteins
  • Oligonucleotides, Antisense
  • Mouth
  • Mesoderm
  • In Situ Hybridization
  • Gene Expression Regulation, Developmental
  • Forkhead Transcription Factors
  • Endoderm
 

Citation

APA
Chicago
ICMJE
MLA
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Oliveri, P., Walton, K. D., Davidson, E. H., & McClay, D. R. (2006). Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo. Development (Cambridge, England), 133(21), 4173–4181. https://doi.org/10.1242/dev.02577
Oliveri, Paola, Katherine D. Walton, Eric H. Davidson, and David R. McClay. “Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo.Development (Cambridge, England) 133, no. 21 (November 2006): 4173–81. https://doi.org/10.1242/dev.02577.
Oliveri P, Walton KD, Davidson EH, McClay DR. Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo. Development (Cambridge, England). 2006 Nov;133(21):4173–81.
Oliveri, Paola, et al. “Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo.Development (Cambridge, England), vol. 133, no. 21, Nov. 2006, pp. 4173–81. Epmc, doi:10.1242/dev.02577.
Oliveri P, Walton KD, Davidson EH, McClay DR. Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo. Development (Cambridge, England). 2006 Nov;133(21):4173–4181.
Journal cover image

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

November 2006

Volume

133

Issue

21

Start / End Page

4173 / 4181

Related Subject Headings

  • Strongylocentrotus purpuratus
  • Signal Transduction
  • Recombinant Proteins
  • Oligonucleotides, Antisense
  • Mouth
  • Mesoderm
  • In Situ Hybridization
  • Gene Expression Regulation, Developmental
  • Forkhead Transcription Factors
  • Endoderm