Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease.
Publication
, Journal Article
Shaunak, S; Albright, RE; Klotman, ME; Henry, SC; Bartlett, JA; Hamilton, JD
Published in: J Infect Dis
June 1990
The polymerase chain reaction (PCR) was used to detect human immunodeficiency virus type 1 (HIV-1) proviral sequences (gag and env) in nucleated cells from the cerebrospinal fluid (CSF) of 31 HIV-1-positive patients, and the results were compared with clinical and radiologic evidence of neurologic disease. Provirus was detected in 21 patients, of whom 20 had neurologic abnormalities. Provirus was not detected in another 6, all of whom were neurologically normal. No neurologic disease has developed in 4 of these 6 patients for whom 12.8 months of follow-up is available. PCR of CSF nucleated cells from HIV-positive patients provides early, rapid, direct evidence of neurologic involvement.
Duke Scholars
Published In
J Infect Dis
DOI
ISSN
0022-1899
Publication Date
June 1990
Volume
161
Issue
6
Start / End Page
1068 / 1072
Location
United States
Related Subject Headings
- Tomography, X-Ray Computed
- Retrospective Studies
- Proviruses
- Polymerase Chain Reaction
- Oligonucleotide Probes
- Nervous System Diseases
- Molecular Sequence Data
- Microbiology
- Male
- Magnetic Resonance Imaging
Citation
APA
Chicago
ICMJE
MLA
NLM
Shaunak, S., Albright, R. E., Klotman, M. E., Henry, S. C., Bartlett, J. A., & Hamilton, J. D. (1990). Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease. J Infect Dis, 161(6), 1068–1072. https://doi.org/10.1093/infdis/161.6.1068
Shaunak, S., R. E. Albright, M. E. Klotman, S. C. Henry, J. A. Bartlett, and J. D. Hamilton. “Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease.” J Infect Dis 161, no. 6 (June 1990): 1068–72. https://doi.org/10.1093/infdis/161.6.1068.
Shaunak S, Albright RE, Klotman ME, Henry SC, Bartlett JA, Hamilton JD. Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease. J Infect Dis. 1990 Jun;161(6):1068–72.
Shaunak, S., et al. “Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease.” J Infect Dis, vol. 161, no. 6, June 1990, pp. 1068–72. Pubmed, doi:10.1093/infdis/161.6.1068.
Shaunak S, Albright RE, Klotman ME, Henry SC, Bartlett JA, Hamilton JD. Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease. J Infect Dis. 1990 Jun;161(6):1068–1072.
Published In
J Infect Dis
DOI
ISSN
0022-1899
Publication Date
June 1990
Volume
161
Issue
6
Start / End Page
1068 / 1072
Location
United States
Related Subject Headings
- Tomography, X-Ray Computed
- Retrospective Studies
- Proviruses
- Polymerase Chain Reaction
- Oligonucleotide Probes
- Nervous System Diseases
- Molecular Sequence Data
- Microbiology
- Male
- Magnetic Resonance Imaging