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Virulence attributes and hyphal growth of C. neoformans are quantitative traits and the MATalpha allele enhances filamentation.

Publication ,  Journal Article
Lin, X; Huang, JC; Mitchell, TG; Heitman, J
Published in: PLoS Genet
November 17, 2006

Cryptococcus neoformans is a fungal human pathogen with a bipolar mating system. It undergoes a dimorphic transition from a unicellular yeast to hyphal filamentous growth during mating and monokaryotic fruiting. The traditional sexual cycle that leads to the production of infectious basidiospores involves cells of both alpha and a mating type. Monokaryotic fruiting is a modified form of sexual reproduction that involves cells of the same mating type, most commonly alpha, which is the predominant mating type in both the environment and clinical isolates. However, some a isolates can also undergo monokaryotic fruiting. To determine whether mating type and other genetic loci contribute to the differences in fruiting observed between alpha and a cells, we applied quantitative trait loci (QTL) mapping to an inbred population of F2 progeny. We discovered that variation in hyphal length produced during fruiting is a quantitative trait resulting from the combined effects of multiple genetic loci, including the mating type (MAT) locus. Importantly, the alpha allele of the MAT locus enhanced hyphal growth compared with the a allele. Other virulence traits, including melanization and growth at 39 degrees C, also are quantitative traits that share a common QTL with hyphal growth. The Mac1 transcription factor, encoded in this common QTL, regulates copper homeostasis. MAC1 allelic differences contribute to phenotypic variation, and mac1Delta mutants exhibit defects in filamentation, melanin production, and high temperature growth. Further characterization of these QTL regions will reveal additional quantitative trait genes controlling biological processes central to fungal development and pathogenicity.

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Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

November 17, 2006

Volume

2

Issue

11

Start / End Page

e187

Location

United States

Related Subject Headings

  • Virulence
  • Temperature
  • Quantitative Trait Loci
  • Molecular Sequence Data
  • Melanins
  • Hyphae
  • Genetic Variation
  • Genetic Markers
  • Genes, Mating Type, Fungal
  • Gene Expression Regulation, Fungal
 

Citation

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Lin, X., Huang, J. C., Mitchell, T. G., & Heitman, J. (2006). Virulence attributes and hyphal growth of C. neoformans are quantitative traits and the MATalpha allele enhances filamentation. PLoS Genet, 2(11), e187. https://doi.org/10.1371/journal.pgen.0020187
Lin, Xiaorong, Johnny C. Huang, Thomas G. Mitchell, and Joseph Heitman. “Virulence attributes and hyphal growth of C. neoformans are quantitative traits and the MATalpha allele enhances filamentation.PLoS Genet 2, no. 11 (November 17, 2006): e187. https://doi.org/10.1371/journal.pgen.0020187.
Lin, Xiaorong, et al. “Virulence attributes and hyphal growth of C. neoformans are quantitative traits and the MATalpha allele enhances filamentation.PLoS Genet, vol. 2, no. 11, Nov. 2006, p. e187. Pubmed, doi:10.1371/journal.pgen.0020187.

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

November 17, 2006

Volume

2

Issue

11

Start / End Page

e187

Location

United States

Related Subject Headings

  • Virulence
  • Temperature
  • Quantitative Trait Loci
  • Molecular Sequence Data
  • Melanins
  • Hyphae
  • Genetic Variation
  • Genetic Markers
  • Genes, Mating Type, Fungal
  • Gene Expression Regulation, Fungal