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alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness.

Publication ,  Journal Article
Lin, X; Litvintseva, AP; Nielsen, K; Patel, S; Floyd, A; Mitchell, TG; Heitman, J
Published in: PLoS Genet
October 2007

Cryptococcus neoformans is a ubiquitous human fungal pathogen that causes meningoencephalitis in predominantly immunocompromised hosts. The fungus is typically haploid, and sexual reproduction involves two individuals with opposite mating types/sexes, alpha and a. However, the overwhelming predominance of mating type (MAT) alpha over a in C. neoformans populations limits alpha-a mating in nature. Recently it was discovered that C. neoformans can undergo same-sex mating under laboratory conditions, especially between alpha isolates. Whether same-sex mating occurs in nature and contributes to the current population structure was unknown. In this study, natural alpha AD alpha hybrids that arose by fusion between two alpha cells of different serotypes (A and D) were identified and characterized, providing definitive evidence that same-sex mating occurs naturally. A novel truncated allele of the mating-type-specific cell identity determinant SXI1 alpha was also identified as a genetic factor likely involved in this process. In addition, laboratory-constructed alpha AD alpha strains exhibited hybrid vigor both in vitro and in vivo, providing a plausible explanation for their relative abundance in nature despite the fact that AD hybrids are inefficient in meiosis/sporulation and are trapped in the diploid state. These findings provide insights on the origins, genetic mechanisms, and fitness impact of unisexual hybridization in the Cryptococcus population.

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Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

October 2007

Volume

3

Issue

10

Start / End Page

1975 / 1990

Location

United States

Related Subject Headings

  • Polymorphism, Genetic
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Models, Genetic
  • Mice
  • Haploidy
  • Genetic Variation
  • Genes, Mating Type, Fungal
  • Diploidy
  • Developmental Biology
 

Citation

APA
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Lin, X., Litvintseva, A. P., Nielsen, K., Patel, S., Floyd, A., Mitchell, T. G., & Heitman, J. (2007). alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness. PLoS Genet, 3(10), 1975–1990. https://doi.org/10.1371/journal.pgen.0030186
Lin, Xiaorong, Anastasia P. Litvintseva, Kirsten Nielsen, Sweta Patel, Anna Floyd, Thomas G. Mitchell, and Joseph Heitman. “alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness.PLoS Genet 3, no. 10 (October 2007): 1975–90. https://doi.org/10.1371/journal.pgen.0030186.
Lin X, Litvintseva AP, Nielsen K, Patel S, Floyd A, Mitchell TG, et al. alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness. PLoS Genet. 2007 Oct;3(10):1975–90.
Lin, Xiaorong, et al. “alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness.PLoS Genet, vol. 3, no. 10, Oct. 2007, pp. 1975–90. Pubmed, doi:10.1371/journal.pgen.0030186.
Lin X, Litvintseva AP, Nielsen K, Patel S, Floyd A, Mitchell TG, Heitman J. alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness. PLoS Genet. 2007 Oct;3(10):1975–1990.

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

October 2007

Volume

3

Issue

10

Start / End Page

1975 / 1990

Location

United States

Related Subject Headings

  • Polymorphism, Genetic
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Models, Genetic
  • Mice
  • Haploidy
  • Genetic Variation
  • Genes, Mating Type, Fungal
  • Diploidy
  • Developmental Biology