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Transcription factor Nrg1 mediates capsule formation, stress response, and pathogenesis in Cryptococcus neoformans.

Publication ,  Journal Article
Cramer, KL; Gerrald, QD; Nichols, CB; Price, MS; Alspaugh, JA
Published in: Eukaryot Cell
July 2006

The Cryptococcus neoformans NRG1 gene was identified using gene microarrays to define putative transcription factor genes regulated by the cyclic AMP (cAMP) signal transduction pathway. Disruption of NRG1 results in delayed capsule formation and mating, two phenotypes that are directly controlled by cAMP signaling. Putative targets of the Nrg1 transcription factor were identified using a second genome microarray to define differences in the transcriptomes of the wild-type and nrg1 mutant strains. These experiments implicate Nrg1 in the transcriptional control of multiple genes involved in carbohydrate metabolism and substrate oxidation, as well as the UGD1 gene encoding a UDP-glucose dehydrogenase required for polysaccharide capsule production and cell wall integrity. In addition to being under transcriptional control of the cAMP pathway, Nrg1 contains a putative protein kinase A phosphorylation site; mutation of this motif results in reduced Nrg1 activity. Consistent with prior studies in hypocapsular mutants, the nrg1 mutant strain is attenuated in an animal model of disseminated cryptococcal disease.

Duke Scholars

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

July 2006

Volume

5

Issue

7

Start / End Page

1147 / 1156

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Saccharomyces cerevisiae Proteins
  • Repressor Proteins
  • Oxidative Stress
  • Organisms, Genetically Modified
  • Oligonucleotide Array Sequence Analysis
  • Mutation
 

Citation

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ICMJE
MLA
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Cramer, K. L., Gerrald, Q. D., Nichols, C. B., Price, M. S., & Alspaugh, J. A. (2006). Transcription factor Nrg1 mediates capsule formation, stress response, and pathogenesis in Cryptococcus neoformans. Eukaryot Cell, 5(7), 1147–1156. https://doi.org/10.1128/EC.00145-06
Cramer, Kari L., Quincy D. Gerrald, Connie B. Nichols, Michael S. Price, and J Andrew Alspaugh. “Transcription factor Nrg1 mediates capsule formation, stress response, and pathogenesis in Cryptococcus neoformans.Eukaryot Cell 5, no. 7 (July 2006): 1147–56. https://doi.org/10.1128/EC.00145-06.
Cramer KL, Gerrald QD, Nichols CB, Price MS, Alspaugh JA. Transcription factor Nrg1 mediates capsule formation, stress response, and pathogenesis in Cryptococcus neoformans. Eukaryot Cell. 2006 Jul;5(7):1147–56.
Cramer, Kari L., et al. “Transcription factor Nrg1 mediates capsule formation, stress response, and pathogenesis in Cryptococcus neoformans.Eukaryot Cell, vol. 5, no. 7, July 2006, pp. 1147–56. Pubmed, doi:10.1128/EC.00145-06.
Cramer KL, Gerrald QD, Nichols CB, Price MS, Alspaugh JA. Transcription factor Nrg1 mediates capsule formation, stress response, and pathogenesis in Cryptococcus neoformans. Eukaryot Cell. 2006 Jul;5(7):1147–1156.

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

July 2006

Volume

5

Issue

7

Start / End Page

1147 / 1156

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Saccharomyces cerevisiae Proteins
  • Repressor Proteins
  • Oxidative Stress
  • Organisms, Genetically Modified
  • Oligonucleotide Array Sequence Analysis
  • Mutation