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Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.

Publication ,  Journal Article
Povsic, TJ; Zavodni, KL; Kelly, FL; Zhu, S; Goldschmidt-Clermont, PJ; Dong, C; Peterson, ED
Published in: J Am Coll Cardiol
December 4, 2007

OBJECTIVES: Our objective was to develop and assess a novel endogenous progenitor cell (EPC) assay based on aldehyde dehydrogenase (ALDH) activity, and to define the relationship of ALDH-bright (ALDH(br)) cells with previously defined EPCs, patient age, and extent of coronary artery disease. BACKGROUND: Accurate assessment of circulating EPCs is of significant interest, yet current assays have limitations. Progenitor cells display high levels of ALDH activity. An assay based on ALDH activity may offer a simple means for enumerating EPCs. METHODS: We simultaneously determined the numbers of EPCs based on ALDH activity and cell surface expression of CD133, CD34, and vascular endothelial growth factor receptor-2 in 110 patients undergoing cardiac catheterization. We assessed the reproducibility of these estimates, correlation among EPC assays, and the association of ALDH(br) numbers with age and disease severity. RESULTS: Aldehyde dehydrogenase-bright cells were easily identified in nonmobilized peripheral blood with median and mean frequencies of 0.041% and 0.074%, respectively. Aldehyde dehydrogenase-bright cells expressed CD34 or CD133 cell surface markers (57.0% and 27.1%, respectively), correlated closely with CD133+CD34+ cells (r = 0.72; p < 0.001), and differentiated into endothelial cells with greater efficiency than CD133+CD34+ cells. Aldehyde dehydrogenase-bright cell numbers were inversely associated with patient age and coronary disease severity. CONCLUSIONS: Aldehyde dehydrogenase activity represents a novel simplified method for quantifying EPCs. The correlation of ALDH(br) cells with clinical factors and outcomes warrants further study.

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Published In

J Am Coll Cardiol

DOI

EISSN

1558-3597

Publication Date

December 4, 2007

Volume

50

Issue

23

Start / End Page

2243 / 2248

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Stem Cells
  • Severity of Illness Index
  • Reproducibility of Results
  • Peptides
  • Middle Aged
  • Male
  • Leukocytes, Mononuclear
  • Humans
  • Glycoproteins
 

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Povsic, T. J., Zavodni, K. L., Kelly, F. L., Zhu, S., Goldschmidt-Clermont, P. J., Dong, C., & Peterson, E. D. (2007). Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity. J Am Coll Cardiol, 50(23), 2243–2248. https://doi.org/10.1016/j.jacc.2007.08.033
Povsic, Thomas J., Katherine L. Zavodni, Francine L. Kelly, Shoukang Zhu, Pascal J. Goldschmidt-Clermont, Chunming Dong, and Eric D. Peterson. “Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.J Am Coll Cardiol 50, no. 23 (December 4, 2007): 2243–48. https://doi.org/10.1016/j.jacc.2007.08.033.
Povsic TJ, Zavodni KL, Kelly FL, Zhu S, Goldschmidt-Clermont PJ, Dong C, et al. Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity. J Am Coll Cardiol. 2007 Dec 4;50(23):2243–8.
Povsic, Thomas J., et al. “Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.J Am Coll Cardiol, vol. 50, no. 23, Dec. 2007, pp. 2243–48. Pubmed, doi:10.1016/j.jacc.2007.08.033.
Povsic TJ, Zavodni KL, Kelly FL, Zhu S, Goldschmidt-Clermont PJ, Dong C, Peterson ED. Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity. J Am Coll Cardiol. 2007 Dec 4;50(23):2243–2248.
Journal cover image

Published In

J Am Coll Cardiol

DOI

EISSN

1558-3597

Publication Date

December 4, 2007

Volume

50

Issue

23

Start / End Page

2243 / 2248

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Stem Cells
  • Severity of Illness Index
  • Reproducibility of Results
  • Peptides
  • Middle Aged
  • Male
  • Leukocytes, Mononuclear
  • Humans
  • Glycoproteins