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Bmp and Fgf signaling are essential for liver specification in zebrafish.

Publication ,  Journal Article
Shin, D; Shin, CH; Tucker, J; Ober, EA; Rentzsch, F; Poss, KD; Hammerschmidt, M; Mullins, MC; Stainier, DYR
Published in: Development
June 2007

Based on data from in vitro tissue explant and ex vivo cell/bead implantation experiments, Bmp and Fgf signaling have been proposed to regulate hepatic specification. However, genetic evidence for this hypothesis has been lacking. Here, we provide in vivo genetic evidence that Bmp and Fgf signaling are essential for hepatic specification. We utilized transgenic zebrafish that overexpress dominant-negative forms of Bmp or Fgf receptors following heat-shock induction. These transgenes allow one to bypass the early embryonic requirements for Bmp and Fgf signaling, and also to completely block Bmp or Fgf signaling. We found that the expression of hhex and prox1, the earliest liver markers in zebrafish, was severely reduced in the liver region when Bmp or Fgf signaling was blocked just before hepatic specification. However, hhex and prox1 expression in adjacent endodermal and mesodermal tissues appeared unaffected by these manipulations. Additional genetic studies indicate that the endoderm maintains competence for Bmp-mediated hepatogenesis over an extended window of embryonic development. Altogether, these data provide the first genetic evidence that Bmp and Fgf signaling are essential for hepatic specification, and suggest that endodermal cells remain competent to differentiate into hepatocytes for longer than anticipated.

Duke Scholars

Published In

Development

DOI

ISSN

0950-1991

Publication Date

June 2007

Volume

134

Issue

11

Start / End Page

2041 / 2050

Location

England

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Tumor Suppressor Proteins
  • Signal Transduction
  • Repressor Proteins
  • Receptors, Fibroblast Growth Factor
  • Morphogenesis
  • Liver
  • In Situ Hybridization
  • Hot Temperature
 

Citation

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MLA
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Shin, D., Shin, C. H., Tucker, J., Ober, E. A., Rentzsch, F., Poss, K. D., … Stainier, D. Y. R. (2007). Bmp and Fgf signaling are essential for liver specification in zebrafish. Development, 134(11), 2041–2050. https://doi.org/10.1242/dev.000281
Shin, Donghun, Chong Hyun Shin, Jennifer Tucker, Elke A. Ober, Fabian Rentzsch, Kenneth D. Poss, Matthias Hammerschmidt, Mary C. Mullins, and Didier Y. R. Stainier. “Bmp and Fgf signaling are essential for liver specification in zebrafish.Development 134, no. 11 (June 2007): 2041–50. https://doi.org/10.1242/dev.000281.
Shin D, Shin CH, Tucker J, Ober EA, Rentzsch F, Poss KD, et al. Bmp and Fgf signaling are essential for liver specification in zebrafish. Development. 2007 Jun;134(11):2041–50.
Shin, Donghun, et al. “Bmp and Fgf signaling are essential for liver specification in zebrafish.Development, vol. 134, no. 11, June 2007, pp. 2041–50. Pubmed, doi:10.1242/dev.000281.
Shin D, Shin CH, Tucker J, Ober EA, Rentzsch F, Poss KD, Hammerschmidt M, Mullins MC, Stainier DYR. Bmp and Fgf signaling are essential for liver specification in zebrafish. Development. 2007 Jun;134(11):2041–2050.
Journal cover image

Published In

Development

DOI

ISSN

0950-1991

Publication Date

June 2007

Volume

134

Issue

11

Start / End Page

2041 / 2050

Location

England

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Tumor Suppressor Proteins
  • Signal Transduction
  • Repressor Proteins
  • Receptors, Fibroblast Growth Factor
  • Morphogenesis
  • Liver
  • In Situ Hybridization
  • Hot Temperature