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Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish.

Publication ,  Journal Article
Yin, VP; Thomson, JM; Thummel, R; Hyde, DR; Hammond, SM; Poss, KD
Published in: Genes Dev
March 15, 2008

Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechanism for complex tissue regeneration.

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

March 15, 2008

Volume

22

Issue

6

Start / End Page

728 / 733

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regeneration
  • Receptors, Fibroblast Growth Factor
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • MicroRNAs
  • Gene Expression Regulation, Developmental
 

Citation

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Yin, V. P., Thomson, J. M., Thummel, R., Hyde, D. R., Hammond, S. M., & Poss, K. D. (2008). Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish. Genes Dev, 22(6), 728–733. https://doi.org/10.1101/gad.1641808
Yin, Viravuth P., J Michael Thomson, Ryan Thummel, David R. Hyde, Scott M. Hammond, and Kenneth D. Poss. “Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish.Genes Dev 22, no. 6 (March 15, 2008): 728–33. https://doi.org/10.1101/gad.1641808.
Yin VP, Thomson JM, Thummel R, Hyde DR, Hammond SM, Poss KD. Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish. Genes Dev. 2008 Mar 15;22(6):728–33.
Yin, Viravuth P., et al. “Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish.Genes Dev, vol. 22, no. 6, Mar. 2008, pp. 728–33. Pubmed, doi:10.1101/gad.1641808.
Yin VP, Thomson JM, Thummel R, Hyde DR, Hammond SM, Poss KD. Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish. Genes Dev. 2008 Mar 15;22(6):728–733.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

March 15, 2008

Volume

22

Issue

6

Start / End Page

728 / 733

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regeneration
  • Receptors, Fibroblast Growth Factor
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • MicroRNAs
  • Gene Expression Regulation, Developmental