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Rat placental lactogen-I binds to the choroid plexus and hypothalamus of the pregnant rat.

Publication ,  Journal Article
Pihoker, C; Robertson, MC; Freemark, M
Published in: J Endocrinol
November 1993

Recent findings suggest that placental lactogen has a role in the regulation of hypothalamic function during pregnancy. To explore the mechanisms by which placental hormones may exert effects in the maternal central nervous system, we have examined the binding of rat placental lactogen-I (rPL-I) to brain slices from pregnant rats at mid- and late gestation. The binding of rPL-I to maternal rat brain was compared with that of human GH (hGH). Radiolabelled rPL-I bound specifically to ependymal cells of the choroid plexus in the lateral ventricles and in the roof of the third ventricle. The binding of 125I-labelled rPL-I was inhibited by unlabelled rPL-I, hGH or rat prolactin but not by rat GH, indicating that rPL-I and rat prolactin interact with a common binding site in maternal rat brain. Radiolabelled hGH bound to the choroid plexus and to ependymal cells lining the third ventricle in the region of the arcuate nucleus. In addition, hGH bound specifically to the ventromedial nuclei and to the medial preoptic area of the hypothalamus. The binding of radiolabelled hGH to all brain regions was inhibited by unlabelled rPL-I as well as hGH, indicating that rPL-I competes for lactogenic binding sites in the hypothalamus as well as the choroid plexus of the pregnant rat. These findings suggest potential mechanisms by which placental hormones may exert direct effects on the maternal central nervous system during pregnancy. The precise functions and roles of the PL-I binding sites in maternal choroid plexus and hypothalamus remain to be explored.

Duke Scholars

Published In

J Endocrinol

DOI

ISSN

0022-0795

Publication Date

November 1993

Volume

139

Issue

2

Start / End Page

235 / 242

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Protein Binding
  • Prolactin
  • Pregnancy, Animal
  • Pregnancy
  • Placental Lactogen
  • In Vitro Techniques
  • Hypothalamus
  • Growth Hormone
 

Citation

APA
Chicago
ICMJE
MLA
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Pihoker, C., Robertson, M. C., & Freemark, M. (1993). Rat placental lactogen-I binds to the choroid plexus and hypothalamus of the pregnant rat. J Endocrinol, 139(2), 235–242. https://doi.org/10.1677/joe.0.1390235
Pihoker, C., M. C. Robertson, and M. Freemark. “Rat placental lactogen-I binds to the choroid plexus and hypothalamus of the pregnant rat.J Endocrinol 139, no. 2 (November 1993): 235–42. https://doi.org/10.1677/joe.0.1390235.
Pihoker C, Robertson MC, Freemark M. Rat placental lactogen-I binds to the choroid plexus and hypothalamus of the pregnant rat. J Endocrinol. 1993 Nov;139(2):235–42.
Pihoker, C., et al. “Rat placental lactogen-I binds to the choroid plexus and hypothalamus of the pregnant rat.J Endocrinol, vol. 139, no. 2, Nov. 1993, pp. 235–42. Pubmed, doi:10.1677/joe.0.1390235.
Pihoker C, Robertson MC, Freemark M. Rat placental lactogen-I binds to the choroid plexus and hypothalamus of the pregnant rat. J Endocrinol. 1993 Nov;139(2):235–242.
Journal cover image

Published In

J Endocrinol

DOI

ISSN

0022-0795

Publication Date

November 1993

Volume

139

Issue

2

Start / End Page

235 / 242

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Protein Binding
  • Prolactin
  • Pregnancy, Animal
  • Pregnancy
  • Placental Lactogen
  • In Vitro Techniques
  • Hypothalamus
  • Growth Hormone