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Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis.

Publication ,  Journal Article
Ishida, S; Huang, E; Zuzan, H; Spang, R; Leone, G; West, M; Nevins, JR
Published in: Mol Cell Biol
July 2001

We have used high-density DNA microarrays to provide an analysis of gene regulation during the mammalian cell cycle and the role of E2F in this process. Cell cycle analysis was facilitated by a combined examination of gene control in serum-stimulated fibroblasts and cells synchronized at G(1)/S by hydroxyurea block that were then released to proceed through the cell cycle. The latter approach (G(1)/S synchronization) is critical for rigorously maintaining cell synchrony for unambiguous analysis of gene regulation in later stages of the cell cycle. Analysis of these samples identified seven distinct clusters of genes that exhibit unique patterns of expression. Genes tend to cluster within these groups based on common function and the time during the cell cycle that the activity is required. Placed in this context, the analysis of genes induced by E2F proteins identified genes or expressed sequence tags not previously described as regulated by E2F proteins; surprisingly, many of these encode proteins known to function during mitosis. A comparison of the E2F-induced genes with the patterns of cell growth-regulated gene expression revealed that virtually all of the E2F-induced genes are found in only two of the cell cycle clusters; one group was regulated at G(1)/S, and the second group, which included the mitotic activities, was regulated at G(2). The activation of the G(2) genes suggests a broader role for E2F in the control of both DNA replication and mitotic activities.

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

July 2001

Volume

21

Issue

14

Start / End Page

4684 / 4699

Location

United States

Related Subject Headings

  • Transcription Factors
  • Retinoblastoma-Binding Protein 1
  • Oligonucleotide Array Sequence Analysis
  • Mitosis
  • Mice
  • Gene Expression Profiling
  • E2F Transcription Factors
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA Replication
 

Citation

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Ishida, S., Huang, E., Zuzan, H., Spang, R., Leone, G., West, M., & Nevins, J. R. (2001). Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis. Mol Cell Biol, 21(14), 4684–4699. https://doi.org/10.1128/MCB.21.14.4684-4699.2001
Ishida, S., E. Huang, H. Zuzan, R. Spang, G. Leone, M. West, and J. R. Nevins. “Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis.Mol Cell Biol 21, no. 14 (July 2001): 4684–99. https://doi.org/10.1128/MCB.21.14.4684-4699.2001.
Ishida S, Huang E, Zuzan H, Spang R, Leone G, West M, et al. Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis. Mol Cell Biol. 2001 Jul;21(14):4684–99.
Ishida, S., et al. “Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis.Mol Cell Biol, vol. 21, no. 14, July 2001, pp. 4684–99. Pubmed, doi:10.1128/MCB.21.14.4684-4699.2001.
Ishida S, Huang E, Zuzan H, Spang R, Leone G, West M, Nevins JR. Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis. Mol Cell Biol. 2001 Jul;21(14):4684–4699.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

July 2001

Volume

21

Issue

14

Start / End Page

4684 / 4699

Location

United States

Related Subject Headings

  • Transcription Factors
  • Retinoblastoma-Binding Protein 1
  • Oligonucleotide Array Sequence Analysis
  • Mitosis
  • Mice
  • Gene Expression Profiling
  • E2F Transcription Factors
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA Replication