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Distinct gene expression phenotypes of cells lacking Rb and Rb family members.

Publication ,  Journal Article
Black, EP; Huang, E; Dressman, H; Rempel, R; Laakso, N; Asa, SL; Ishida, S; West, M; Nevins, JR
Published in: Cancer Res
July 1, 2003

The study of tumor suppressor gene function has been aided by the creation of discrete gene alterations in the mouse. One such example can be seen in the study of tumor suppressor gene function in general and the retinoblastoma (Rb) tumor suppressor in particular. Because the phenotype of a cell is a direct reflection of the gene activity within that cell, a comprehensive analysis of changes in gene activity resulting from the loss of Rb function has the potential to greatly enhance our understanding of Rb biology. We have used DNA microarray analysis to identify gene expression profiles in wild-type and Rb-null mouse embryo fibroblasts, as well as cells lacking other Rb family members, as an approach to developing a more complete understanding of Rb function. In so doing, we have identified gene expression phenotypes that characterize the loss of Rb function, that distinguish a Rb-null cell from a wild-type cell as well as a p107/p130-null cell, and that identify gene regulatory pathways unique to these events. Importantly, the Rb gene expression patterns can identify murine tumors that result from Rb loss of function. We suggest that this is an approach to the eventual understanding of gene regulatory pathways that define a phenotypic state, including those events that lead to tumor development.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

July 1, 2003

Volume

63

Issue

13

Start / End Page

3716 / 3723

Location

United States

Related Subject Headings

  • Retinoblastoma Protein
  • Phenotype
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Genes, Retinoblastoma
  • Gene Expression Regulation
  • Gene Deletion
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Black, E. P., Huang, E., Dressman, H., Rempel, R., Laakso, N., Asa, S. L., … Nevins, J. R. (2003). Distinct gene expression phenotypes of cells lacking Rb and Rb family members. Cancer Res, 63(13), 3716–3723.
Black, Esther P., Erich Huang, Holly Dressman, Rachel Rempel, Nina Laakso, Sylvia L. Asa, Seiichi Ishida, Mike West, and Joseph R. Nevins. “Distinct gene expression phenotypes of cells lacking Rb and Rb family members.Cancer Res 63, no. 13 (July 1, 2003): 3716–23.
Black EP, Huang E, Dressman H, Rempel R, Laakso N, Asa SL, et al. Distinct gene expression phenotypes of cells lacking Rb and Rb family members. Cancer Res. 2003 Jul 1;63(13):3716–23.
Black, Esther P., et al. “Distinct gene expression phenotypes of cells lacking Rb and Rb family members.Cancer Res, vol. 63, no. 13, July 2003, pp. 3716–23.
Black EP, Huang E, Dressman H, Rempel R, Laakso N, Asa SL, Ishida S, West M, Nevins JR. Distinct gene expression phenotypes of cells lacking Rb and Rb family members. Cancer Res. 2003 Jul 1;63(13):3716–3723.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

July 1, 2003

Volume

63

Issue

13

Start / End Page

3716 / 3723

Location

United States

Related Subject Headings

  • Retinoblastoma Protein
  • Phenotype
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Genes, Retinoblastoma
  • Gene Expression Regulation
  • Gene Deletion