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Role of vascular mitogens in subarachnoid hemorrhage-associated cerebral vasculopathy.

Publication ,  Journal Article
Miller, CA; Lombard, FW; Wu, C-T; Hubbard, CJ; Silbajoris, L; Borel, CO; Niklason, LE
Published in: Neurocrit Care
2006

INTRODUCTION: Cerebral vasculopathy may play an important role in the development of delayed cerebral ischemia following subarachnoid hemorrhage (SAH). Platelet-derived growth factor AB (PDGF-AB) and vascular endothelial growth factor (VEGF) released from blood clot may trigger vasculopathy in cerebral arteries. We compared arteriographic and histological response to injection of blood with PDGF-AB and VEGF in basilar artery over 3 days. METHODS: A total of 55 male New Zealand white rabbits were used in this study. SAH was simulated by single injection of 1 ml of autologous blood, while 50 microg PDGF-AB, 100 microg VEGF, and 50 microg PDGF-AB/100 microg VEGF combined were given in autologous cerebrospinal fluid (CSF) into the cisterna magna. Eight rabbits served as controls, receiving arteriograms but no cisternal injections, and 13 rabbits received reinjections of CSF. Basilar artery diameter was measured arteriographically at baseline and 3 days after injection. Immunohistochemistry was performed to assess change in the basilar artery. RESULTS: Control groups showed significantly less (p < 0.0002) basilar artery narrowing than all treatment groups. Proliferating cell nuclear antigen (PCNA) was not significant for treatment groups compared to CSF reinject control. PCNA was significantly different for the no puncture group compared to the CSF reinject control. Von Willebrand Factor staining may indicate endothelial proliferation in the adventitia of SAH, VEGF, and PDGFAB/ VEGF combined groups. PDGF labeling was abundant for SAH, PDGF-AB, and PGDF-AB/VEGF combined. CONCLUSIONS: PDGF-AB/VEGF and VEGF cause narrowing of the basilar artery similar to cisternal blood clot at 3 days, and thus blood clot was not required to cause arteriographic changes consistent with cerebral vasculopathy.

Duke Scholars

Published In

Neurocrit Care

DOI

ISSN

1541-6933

Publication Date

2006

Volume

5

Issue

3

Start / End Page

215 / 221

Location

United States

Related Subject Headings

  • Vasospasm, Intracranial
  • Vascular Resistance
  • Vascular Endothelial Growth Factor A
  • Subarachnoid Hemorrhage
  • Rabbits
  • Platelet-Derived Growth Factor
  • Neurology & Neurosurgery
  • Muscle, Smooth, Vascular
  • Male
  • Endothelium, Vascular
 

Citation

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ICMJE
MLA
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Miller, C. A., Lombard, F. W., Wu, C.-T., Hubbard, C. J., Silbajoris, L., Borel, C. O., & Niklason, L. E. (2006). Role of vascular mitogens in subarachnoid hemorrhage-associated cerebral vasculopathy. Neurocrit Care, 5(3), 215–221. https://doi.org/10.1385/NCC:5:3:215
Miller, Cheryl A., Frederick W. Lombard, Ching-Tang Wu, Cory J. Hubbard, Lydia Silbajoris, Cecil O. Borel, and Laura E. Niklason. “Role of vascular mitogens in subarachnoid hemorrhage-associated cerebral vasculopathy.Neurocrit Care 5, no. 3 (2006): 215–21. https://doi.org/10.1385/NCC:5:3:215.
Miller CA, Lombard FW, Wu C-T, Hubbard CJ, Silbajoris L, Borel CO, et al. Role of vascular mitogens in subarachnoid hemorrhage-associated cerebral vasculopathy. Neurocrit Care. 2006;5(3):215–21.
Miller, Cheryl A., et al. “Role of vascular mitogens in subarachnoid hemorrhage-associated cerebral vasculopathy.Neurocrit Care, vol. 5, no. 3, 2006, pp. 215–21. Pubmed, doi:10.1385/NCC:5:3:215.
Miller CA, Lombard FW, Wu C-T, Hubbard CJ, Silbajoris L, Borel CO, Niklason LE. Role of vascular mitogens in subarachnoid hemorrhage-associated cerebral vasculopathy. Neurocrit Care. 2006;5(3):215–221.
Journal cover image

Published In

Neurocrit Care

DOI

ISSN

1541-6933

Publication Date

2006

Volume

5

Issue

3

Start / End Page

215 / 221

Location

United States

Related Subject Headings

  • Vasospasm, Intracranial
  • Vascular Resistance
  • Vascular Endothelial Growth Factor A
  • Subarachnoid Hemorrhage
  • Rabbits
  • Platelet-Derived Growth Factor
  • Neurology & Neurosurgery
  • Muscle, Smooth, Vascular
  • Male
  • Endothelium, Vascular