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Newspapers and newspaper ink contain agonists for the ah receptor.

Publication ,  Journal Article
Bohonowych, JES; Zhao, B; Timme-Laragy, A; Jung, D; Di Giulio, RT; Denison, MS
Published in: Toxicological sciences : an official journal of the Society of Toxicology
April 2008

Ligand-dependent activation of the aryl hydrocarbon receptor (AhR) pathway leads to a diverse array of biological and toxicological effects. The best-studied ligands for the AhR include polycyclic and halogenated aromatic hydrocarbons, the most potent of which is 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). However, as new AhR ligands are identified and characterized, their structural and physiochemical diversity continues to expand. Our identification of AhR agonists in crude extracts from diverse materials raises questions as to the magnitude and extent of human exposure to AhR ligands through normal daily activities. We have found that solvent extracts of newspapers from countries around the world stimulate the AhR signaling pathway. AhR agonist activity was observed for dimethyl sulfoxide (DMSO), ethanol, and water extracts of printed newspaper, unprinted virgin paper, and black printing ink, where activation of luciferase reporter gene expression was transient, suggesting that the AhR active chemical(s) was metabolically labile. DMSO and ethanol extracts also stimulated AhR transformation and DNA binding, and also competed with [(3)H]TCDD for binding to the AhR. In addition, DMSO extracts of printed newspaper induced cytochrome P450 1A associated 7-ethoxyresorufin-O-deethylase activity in zebrafish embryos in vivo. Although the responsible bioactive chemical(s) remain to be identified, our results demonstrate that newspapers and printing ink contain relatively potent metabolically labile agonists of the AhR. Given the large amount of recycling and reprocessing of newspapers throughout the world, release of these easily extractable AhR agonists into the environment should be examined and their potential effects on aquatic organisms assessed.

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Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

April 2008

Volume

102

Issue

2

Start / End Page

278 / 290

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Solvents
  • Signal Transduction
  • Receptors, Aryl Hydrocarbon
  • Polychlorinated Dibenzodioxins
  • Newspapers as Topic
  • Male
  • Luciferases
  • Ligands
 

Citation

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Bohonowych, J. E. S., Zhao, B., Timme-Laragy, A., Jung, D., Di Giulio, R. T., & Denison, M. S. (2008). Newspapers and newspaper ink contain agonists for the ah receptor. Toxicological Sciences : An Official Journal of the Society of Toxicology, 102(2), 278–290. https://doi.org/10.1093/toxsci/kfn011
Bohonowych, Jessica E. S., Bin Zhao, Alicia Timme-Laragy, Dawoon Jung, Richard T. Di Giulio, and Michael S. Denison. “Newspapers and newspaper ink contain agonists for the ah receptor.Toxicological Sciences : An Official Journal of the Society of Toxicology 102, no. 2 (April 2008): 278–90. https://doi.org/10.1093/toxsci/kfn011.
Bohonowych JES, Zhao B, Timme-Laragy A, Jung D, Di Giulio RT, Denison MS. Newspapers and newspaper ink contain agonists for the ah receptor. Toxicological sciences : an official journal of the Society of Toxicology. 2008 Apr;102(2):278–90.
Bohonowych, Jessica E. S., et al. “Newspapers and newspaper ink contain agonists for the ah receptor.Toxicological Sciences : An Official Journal of the Society of Toxicology, vol. 102, no. 2, Apr. 2008, pp. 278–90. Epmc, doi:10.1093/toxsci/kfn011.
Bohonowych JES, Zhao B, Timme-Laragy A, Jung D, Di Giulio RT, Denison MS. Newspapers and newspaper ink contain agonists for the ah receptor. Toxicological sciences : an official journal of the Society of Toxicology. 2008 Apr;102(2):278–290.
Journal cover image

Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

April 2008

Volume

102

Issue

2

Start / End Page

278 / 290

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Solvents
  • Signal Transduction
  • Receptors, Aryl Hydrocarbon
  • Polychlorinated Dibenzodioxins
  • Newspapers as Topic
  • Male
  • Luciferases
  • Ligands