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Rapid detection of the Fc gamma RIIA-H/R 131 ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED).
Publication
, Journal Article
Jiang, XM; Arepally, G; Poncz, M; McKenzie, SE
Published in: J Immunol Methods
November 29, 1996
A polymorphism of the gene for Fc gamma RIIA, arginine (R) or histidine (H) at position 131, alters the ability of the receptor to bind certain IgG subclasses. Identification of the Fc gamma RIIA-H/R 131 genotype has assumed increasing importance in disorders of host defense, immunohematologic diseases and systemic autoimmune disorders. We report a new method for determination of this genotype in which an allele-specific restriction enzyme site is introduced into an Fc gamma RIIA PCR product from genomic DNA, and polymorphism assignment is determined by restriction enzyme digestion followed by agarose gel electrophoresis. This method is more rapid, more reliable and less expensive than currently available methods.
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Published In
J Immunol Methods
DOI
ISSN
0022-1759
Publication Date
November 29, 1996
Volume
199
Issue
1
Start / End Page
55 / 59
Location
Netherlands
Related Subject Headings
- Receptors, IgG
- Polymorphism, Genetic
- Ligands
- Immunology
- Humans
- Histidine
- DNA Restriction Enzymes
- Arginine
- Alleles
- 3204 Immunology
Citation
APA
Chicago
ICMJE
MLA
NLM
Jiang, X. M., Arepally, G., Poncz, M., & McKenzie, S. E. (1996). Rapid detection of the Fc gamma RIIA-H/R 131 ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED). J Immunol Methods, 199(1), 55–59. https://doi.org/10.1016/s0022-1759(96)00164-0
Jiang, X. M., G. Arepally, M. Poncz, and S. E. McKenzie. “Rapid detection of the Fc gamma RIIA-H/R 131 ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED).” J Immunol Methods 199, no. 1 (November 29, 1996): 55–59. https://doi.org/10.1016/s0022-1759(96)00164-0.
Jiang XM, Arepally G, Poncz M, McKenzie SE. Rapid detection of the Fc gamma RIIA-H/R 131 ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED). J Immunol Methods. 1996 Nov 29;199(1):55–9.
Jiang, X. M., et al. “Rapid detection of the Fc gamma RIIA-H/R 131 ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED).” J Immunol Methods, vol. 199, no. 1, Nov. 1996, pp. 55–59. Pubmed, doi:10.1016/s0022-1759(96)00164-0.
Jiang XM, Arepally G, Poncz M, McKenzie SE. Rapid detection of the Fc gamma RIIA-H/R 131 ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED). J Immunol Methods. 1996 Nov 29;199(1):55–59.
![Journal cover image](https://secure.syndetics.com/index.aspx?isbn=/lc.gif&issn=0022-1759&client=dukeuniv)
Published In
J Immunol Methods
DOI
ISSN
0022-1759
Publication Date
November 29, 1996
Volume
199
Issue
1
Start / End Page
55 / 59
Location
Netherlands
Related Subject Headings
- Receptors, IgG
- Polymorphism, Genetic
- Ligands
- Immunology
- Humans
- Histidine
- DNA Restriction Enzymes
- Arginine
- Alleles
- 3204 Immunology