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Localization of a cryptic binding site for tenascin on fibronectin.

Publication ,  Journal Article
Ingham, KC; Brew, SA; Erickson, HP
Published in: J Biol Chem
July 2, 2004

Fibronectin and tenascin are large extracellular matrix proteins that interact with each other and with integrin receptors to regulate cell growth and movement. They are both modular proteins composed of independently folded domains (modules) that are arranged in linear fashion. Fibronectin is a covalent dimer and tenascin is a hexamer. The site on tenascin to which fibronectin binds has been localized to type III modules 3-5. In this study we use surface plasmon resonance to examine the interaction between various fragments of fibronectin and tenascin to further characterize and localize the binding sites. We found that tenascin fragments that contain type III modules 3-5 bind primarily to the N-terminal 29-kDa hep-1/fib-1 domain, which contains the first five type I modules of fibronectin. The dissociation constant, K(d), is approximately 1 microm. The binding site on fibronectin appears to be cryptic in the whole molecule in solution but is exposed on the proteolytic fragments and probably when fibronectin is in the extended conformation.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 2, 2004

Volume

279

Issue

27

Start / End Page

28132 / 28135

Location

United States

Related Subject Headings

  • Time Factors
  • Tenascin
  • Temperature
  • Swine
  • Surface Plasmon Resonance
  • Spectrophotometry
  • Protein Structure, Tertiary
  • Protein Folding
  • Protein Conformation
  • Protein Binding
 

Citation

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Ingham, K. C., Brew, S. A., & Erickson, H. P. (2004). Localization of a cryptic binding site for tenascin on fibronectin. J Biol Chem, 279(27), 28132–28135. https://doi.org/10.1074/jbc.M312785200
Ingham, Kenneth C., Shelesa A. Brew, and Harold P. Erickson. “Localization of a cryptic binding site for tenascin on fibronectin.J Biol Chem 279, no. 27 (July 2, 2004): 28132–35. https://doi.org/10.1074/jbc.M312785200.
Ingham KC, Brew SA, Erickson HP. Localization of a cryptic binding site for tenascin on fibronectin. J Biol Chem. 2004 Jul 2;279(27):28132–5.
Ingham, Kenneth C., et al. “Localization of a cryptic binding site for tenascin on fibronectin.J Biol Chem, vol. 279, no. 27, July 2004, pp. 28132–35. Pubmed, doi:10.1074/jbc.M312785200.
Ingham KC, Brew SA, Erickson HP. Localization of a cryptic binding site for tenascin on fibronectin. J Biol Chem. 2004 Jul 2;279(27):28132–28135.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 2, 2004

Volume

279

Issue

27

Start / End Page

28132 / 28135

Location

United States

Related Subject Headings

  • Time Factors
  • Tenascin
  • Temperature
  • Swine
  • Surface Plasmon Resonance
  • Spectrophotometry
  • Protein Structure, Tertiary
  • Protein Folding
  • Protein Conformation
  • Protein Binding