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In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function.

Publication ,  Journal Article
Stricker, J; Erickson, HP
Published in: J Bacteriol
August 2003

We have characterized the in vivo phenotypes of 17 mutations of Escherichia coli ftsZ. In particular, we determined whether these mutations can complement a null ftsZ phenotype, and we demonstrated that two noncomplementing mutations show partial dominant-negative behavior. We performed immunofluorescence microscopy to determine whether these mutants could assemble into normal or abnormal structures in vivo. The mutants separated into four classes-those that complemented the null and formed normal FtsZ rings, those that complemented the null but formed aberrant FtsZ structures, those that formed aberrant FtsZ structures and did not complement, and those that were unable to form any FtsZ structures. We did not find any mutations that produced nonfunctional Z rings of normal appearance. Surprisingly, some mutants that produced extensively spiraled Z-ring structures divided and grew with a normal doubling time. The analysis was carried out using a complementation system based on an ftsZ deletion strain, a temperature-sensitive rescue plasmid, and a complementation vector that placed mutated ftsZ alleles under the control of the pBAD promoter, which offered several advantages over previous systems.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

August 2003

Volume

185

Issue

16

Start / End Page

4796 / 4805

Location

United States

Related Subject Headings

  • Mutation
  • Microscopy, Fluorescence
  • Microbiology
  • Genetic Complementation Test
  • Escherichia coli
  • Cytoskeletal Proteins
  • Culture Media
  • Cell Division
  • Bacterial Proteins
  • 32 Biomedical and clinical sciences
 

Citation

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Stricker, J., & Erickson, H. P. (2003). In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function. J Bacteriol, 185(16), 4796–4805. https://doi.org/10.1128/JB.185.16.4796-4805.2003
Stricker, Jesse, and Harold P. Erickson. “In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function.J Bacteriol 185, no. 16 (August 2003): 4796–4805. https://doi.org/10.1128/JB.185.16.4796-4805.2003.
Stricker J, Erickson HP. In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function. J Bacteriol. 2003 Aug;185(16):4796–805.
Stricker, Jesse, and Harold P. Erickson. “In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function.J Bacteriol, vol. 185, no. 16, Aug. 2003, pp. 4796–805. Pubmed, doi:10.1128/JB.185.16.4796-4805.2003.
Stricker J, Erickson HP. In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function. J Bacteriol. 2003 Aug;185(16):4796–4805.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

August 2003

Volume

185

Issue

16

Start / End Page

4796 / 4805

Location

United States

Related Subject Headings

  • Mutation
  • Microscopy, Fluorescence
  • Microbiology
  • Genetic Complementation Test
  • Escherichia coli
  • Cytoskeletal Proteins
  • Culture Media
  • Cell Division
  • Bacterial Proteins
  • 32 Biomedical and clinical sciences