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Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta.

Publication ,  Journal Article
Morikawa, M; Fryer, JD; Sullivan, PM; Christopher, EA; Wahrle, SE; DeMattos, RB; O'Dell, MA; Fagan, AM; Lashuel, HA; Walz, T; Asai, K; Holtzman, DM
Published in: Neurobiol Dis
2005

The apolipoprotein E (apoE) genotype is an important genetic risk factor for Alzheimer's disease (AD). In the central nervous system (CNS), most apoE is produced by astrocytes and is present in unique high-density lipoprotein (HDL)-like particles that have distinct properties from apoE derived from other sources. To develop an efficient system to produce astrocyte-derived apoE in large quantities, we produced and characterized immortalized cell lines from primary astrocyte cultures derived from human APOE knock-in mice. APOE2, APOE3, and APOE4 expressing cell lines were established that secrete apoE in HDL-like particles at similar levels, cholesterol composition, and size as those produced by primary astrocytes. In physiological buffers, astrocyte-secreted apoE3 and E4 associated equally well with amyloid-beta. Under the same conditions, only a small fraction of A beta formed sodium dodecyl sulfate (SDS)-stable complexes with apoE (E3 > E4). These immortalized astrocytes will be useful for studying mechanisms underlying the isoform-specific effects of apoE in the CNS.

Duke Scholars

Published In

Neurobiol Dis

DOI

ISSN

0969-9961

Publication Date

2005

Volume

19

Issue

1-2

Start / End Page

66 / 76

Location

United States

Related Subject Headings

  • Protein Isoforms
  • Neurons
  • Neurology & Neurosurgery
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Humans
  • Hippocampus
  • Cells, Cultured
  • Cell Line, Transformed
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Morikawa, M., Fryer, J. D., Sullivan, P. M., Christopher, E. A., Wahrle, S. E., DeMattos, R. B., … Holtzman, D. M. (2005). Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta. Neurobiol Dis, 19(1–2), 66–76. https://doi.org/10.1016/j.nbd.2004.11.005
Morikawa, Masayuki, John D. Fryer, Patrick M. Sullivan, Erin A. Christopher, Suzanne E. Wahrle, Ronald B. DeMattos, Mark A. O’Dell, et al. “Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta.Neurobiol Dis 19, no. 1–2 (2005): 66–76. https://doi.org/10.1016/j.nbd.2004.11.005.
Morikawa M, Fryer JD, Sullivan PM, Christopher EA, Wahrle SE, DeMattos RB, et al. Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta. Neurobiol Dis. 2005;19(1–2):66–76.
Morikawa, Masayuki, et al. “Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta.Neurobiol Dis, vol. 19, no. 1–2, 2005, pp. 66–76. Pubmed, doi:10.1016/j.nbd.2004.11.005.
Morikawa M, Fryer JD, Sullivan PM, Christopher EA, Wahrle SE, DeMattos RB, O’Dell MA, Fagan AM, Lashuel HA, Walz T, Asai K, Holtzman DM. Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta. Neurobiol Dis. 2005;19(1–2):66–76.
Journal cover image

Published In

Neurobiol Dis

DOI

ISSN

0969-9961

Publication Date

2005

Volume

19

Issue

1-2

Start / End Page

66 / 76

Location

United States

Related Subject Headings

  • Protein Isoforms
  • Neurons
  • Neurology & Neurosurgery
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Humans
  • Hippocampus
  • Cells, Cultured
  • Cell Line, Transformed