Scholars@Duke publication: Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology.

Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology.

Publication , Journal Article

LoMonaco, MB; Batzer, MA; Tedeschi, B; Chang, SH; Lee, WR

Published in: Mutat Res

February 1988

An alcohol dehydrogenase (ADH) null mutant of Drosophila melanogaster (AdhnLA405) originally recovered following X-ray irradiation of mature sperm (Aaron, 979) is analyzed by Southern blotting, Western blotting, and DNA sequencing. The genetic, immunologic, and nucleic acid sequence data are consistent with the hypothesis that a cross-over event, independent of X-irradiation, between parental chromosomes is responsible for the ADH null phenotype of AdhnLA405. By DNA-sequence analysis we show that molecular cloning of this locus (i.e., propagation in prokaryotic hosts) apparently does not introduce any spurious changes (substitutions, additions, deletions, or rearrangements) within the DNA.

Duke Scholars

Author W. Robert Lee Radiation Oncology

Published In

Mutat Res

DOI

10.1016/0165-7992(88)90041-3

ISSN

0027-5107

Publication Date

February 1988

Volume

207

Issue

Start / End Page

53 / 56

Location

Netherlands

Related Subject Headings

Oncology & Carcinogenesis
Mutation
Drosophila melanogaster
DNA
Base Sequence
Animals
Alcohol Dehydrogenase
3105 Genetics

Citation

APA

Chicago

ICMJE

MLA

NLM

LoMonaco, M. B., Batzer, M. A., Tedeschi, B., Chang, S. H., & Lee, W. R. (1988). Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology. Mutat Res, 207(2), 53–56. https://doi.org/10.1016/0165-7992(88)90041-3

LoMonaco, M. B., M. A. Batzer, B. Tedeschi, S. H. Chang, and W. R. Lee. “Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology.” Mutat Res 207, no. 2 (February 1988): 53–56. https://doi.org/10.1016/0165-7992(88)90041-3.

LoMonaco MB, Batzer MA, Tedeschi B, Chang SH, Lee WR. Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology. Mutat Res. 1988 Feb;207(2):53–6.

LoMonaco, M. B., et al. “Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology.” Mutat Res, vol. 207, no. 2, Feb. 1988, pp. 53–56. Pubmed, doi:10.1016/0165-7992(88)90041-3.

LoMonaco MB, Batzer MA, Tedeschi B, Chang SH, Lee WR. Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology. Mutat Res. 1988 Feb;207(2):53–56.