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The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies.

Publication ,  Journal Article
Shen, HD; Choo, KB; Lee, HH; Hsieh, JC; Lin, WL; Lee, WR; Han, SH
Published in: Clin Exp Allergy
November 1991

To characterize the 40-kilodalton (kD) major allergen of Candida albicans (C. albicans), six monoclonal antibodies (MoAbs) against this allergen were generated. In SDS-polyacrylamide gel electrophoresis and immunoblot analysis, these MoAbs showed four different reaction patterns to antigens of six different Candida species. With the exception of one MoAb, other MoAbs were resistant to periodate treatment indicating non-carbohydrate epitopes were probably being recognized by these MoAbs. These MoAbs were used in the molecular cloning and immunological analysis of the gene coding for the 40-kD allergen. Nucleotide sequence determination of the two lambda gt11 cDNA clones obtained showed that the 40-kD allergen is an alcohol dehydrogenase (ADH) which shares a 70% amino acid sequence homology with the ADH isozyme I of Saccharomyces cerevisiae. This finding was confirmed by positive immunological response of the lysates of the clones obtained and a preparation of ADH of Saccharomyces cerevisiae to various MoAbs and to IgE antibodies in sera of allergic patients.

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Published In

Clin Exp Allergy

DOI

ISSN

0954-7894

Publication Date

November 1991

Volume

21

Issue

6

Start / End Page

675 / 681

Location

England

Related Subject Headings

  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Molecular Sequence Data
  • Mice, Inbred BALB C
  • Mice
  • Genes, Fungal
  • Fungal Proteins
  • DNA, Fungal
  • DNA
  • Cloning, Molecular
 

Citation

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Shen, H. D., Choo, K. B., Lee, H. H., Hsieh, J. C., Lin, W. L., Lee, W. R., & Han, S. H. (1991). The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies. Clin Exp Allergy, 21(6), 675–681. https://doi.org/10.1111/j.1365-2222.1991.tb03195.x
Shen, H. D., K. B. Choo, H. H. Lee, J. C. Hsieh, W. L. Lin, W. R. Lee, and S. H. Han. “The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies.Clin Exp Allergy 21, no. 6 (November 1991): 675–81. https://doi.org/10.1111/j.1365-2222.1991.tb03195.x.
Shen HD, Choo KB, Lee HH, Hsieh JC, Lin WL, Lee WR, et al. The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies. Clin Exp Allergy. 1991 Nov;21(6):675–81.
Shen, H. D., et al. “The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies.Clin Exp Allergy, vol. 21, no. 6, Nov. 1991, pp. 675–81. Pubmed, doi:10.1111/j.1365-2222.1991.tb03195.x.
Shen HD, Choo KB, Lee HH, Hsieh JC, Lin WL, Lee WR, Han SH. The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies. Clin Exp Allergy. 1991 Nov;21(6):675–681.
Journal cover image

Published In

Clin Exp Allergy

DOI

ISSN

0954-7894

Publication Date

November 1991

Volume

21

Issue

6

Start / End Page

675 / 681

Location

England

Related Subject Headings

  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Molecular Sequence Data
  • Mice, Inbred BALB C
  • Mice
  • Genes, Fungal
  • Fungal Proteins
  • DNA, Fungal
  • DNA
  • Cloning, Molecular