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Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries.

Publication ,  Journal Article
Lan, MA; Gersbach, CA; Michael, KE; Keselowsky, BG; García, AJ
Published in: Biomaterials
August 2005

Biomaterial surface properties modulate protein adsorption and cell adhesion to elicit diverse cellular responses in biomedical and biotechnological applications. We used alkanethiol self-assembled monolayers presenting well-defined chemistries (OH, CH(3), NH(2), and COOH) to analyze the effects of surface chemistry on myoblast proliferation and differentiation. Surfaces were pre-coated with equivalent densities of fibronectin. C2C12 skeletal myoblasts exhibited surface-dependent differences in cell proliferation (COOH = NH(2) > CH(3) = OH). Myogenin and troponin T gene expression levels were up-regulated on CH(3) and OH surfaces compared to other chemistries. Furthermore, immunostaining for sarcomeric myosin revealed surface chemistry-dependent differences in myogenic differentiation following the pattern OH > CH(3) > NH(2) = COOH. Immunostaining analyses of integrin subunits demonstrated surface chemistry-dependent differences in integrin binding to adsorbed fibronectin. OH and CH(3) surfaces supported selective binding of alpha(5)beta(1) integrin while the COOH and NH(2) functionalities displayed binding of both alpha(5)beta(1) and alpha(V)beta(3) Myogenic differentiation correlated with differences in integrin binding; surface chemistries that supported selective binding of alpha(5)beta(1) displayed enhanced differentiation. Finally, blocking beta(1), but not beta(3), integrins inhibited differentiation, implicating specific integrins in the differentiation process. These results demonstrate that surface chemistry modulates myoblast proliferation and differentiation via differences in integrin binding to adsorbed fibronectin.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

August 2005

Volume

26

Issue

22

Start / End Page

4523 / 4531

Related Subject Headings

  • Troponin T
  • Surface Properties
  • Reverse Transcriptase Polymerase Chain Reaction
  • Myogenin
  • Myoblasts
  • Mice
  • Integrins
  • Gene Expression Regulation
  • Fibronectins
  • Cell Proliferation
 

Citation

APA
Chicago
ICMJE
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Lan, M. A., Gersbach, C. A., Michael, K. E., Keselowsky, B. G., & García, A. J. (2005). Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries. Biomaterials, 26(22), 4523–4531. https://doi.org/10.1016/j.biomaterials.2004.11.028
Lan, Michael A., Charles A. Gersbach, Kristin E. Michael, Benjamin G. Keselowsky, and Andrés J. García. “Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries.Biomaterials 26, no. 22 (August 2005): 4523–31. https://doi.org/10.1016/j.biomaterials.2004.11.028.
Lan MA, Gersbach CA, Michael KE, Keselowsky BG, García AJ. Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries. Biomaterials. 2005 Aug;26(22):4523–31.
Lan, Michael A., et al. “Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries.Biomaterials, vol. 26, no. 22, Aug. 2005, pp. 4523–31. Epmc, doi:10.1016/j.biomaterials.2004.11.028.
Lan MA, Gersbach CA, Michael KE, Keselowsky BG, García AJ. Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries. Biomaterials. 2005 Aug;26(22):4523–4531.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

August 2005

Volume

26

Issue

22

Start / End Page

4523 / 4531

Related Subject Headings

  • Troponin T
  • Surface Properties
  • Reverse Transcriptase Polymerase Chain Reaction
  • Myogenin
  • Myoblasts
  • Mice
  • Integrins
  • Gene Expression Regulation
  • Fibronectins
  • Cell Proliferation