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Biomaterial-mediated retroviral gene transfer using self-assembled monolayers.

Publication ,  Journal Article
Gersbach, CA; Coyer, SR; Le Doux, JM; García, AJ
Published in: Biomaterials
December 2007

Biomaterial-mediated gene delivery has recently emerged as a promising alternative to conventional gene transfer technologies that focus on direct delivery of viral vectors or DNA-polymer/matrix complexes. However, biomaterial-based strategies have primarily targeted transient gene expression vehicles, including plasmid DNA and adenovirus particles. This study expands on this work by characterizing biomaterial properties conducive to the surface immobilization of retroviral particles and subsequent transduction of mammalian cells at the cell-material interface. Self-assembled monolayers (SAMs) of functionally-terminated alkanethiols on gold were used to establish biomaterial surfaces of defined chemical composition. Gene transfer was observed to be greater than 90% on NH(2)-terminated surfaces, approximately 50% on COOH-functionalized surfaces, and undetectable on CH(3)-terminated SAMs, similar to controls of tissue culture-treated polystyrene. Gene delivery via the NH(2)-SAM was further characterized as a function of retrovirus coating time, virus concentration, and cell seeding density. Finally, SAM-mediated gene delivery was comparable to fibronectin- and poly-l-lysine-based methods for gene transfer. This work is significant to establishing safe and effective gene therapy strategies, developing efficient methods for gene delivery, and supporting recent progress in the field of biomaterial-mediated gene transfer.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

December 2007

Volume

28

Issue

34

Start / End Page

5121 / 5127

Related Subject Headings

  • Retroviridae
  • Polymers
  • Polylysine
  • Plasmids
  • NIH 3T3 Cells
  • Microscopy, Electron, Scanning
  • Mice
  • Genetic Therapy
  • Genetic Engineering
  • Gene Transfer Techniques
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gersbach, C. A., Coyer, S. R., Le Doux, J. M., & García, A. J. (2007). Biomaterial-mediated retroviral gene transfer using self-assembled monolayers. Biomaterials, 28(34), 5121–5127. https://doi.org/10.1016/j.biomaterials.2007.07.047
Gersbach, Charles A., Sean R. Coyer, Joseph M. Le Doux, and Andrés J. García. “Biomaterial-mediated retroviral gene transfer using self-assembled monolayers.Biomaterials 28, no. 34 (December 2007): 5121–27. https://doi.org/10.1016/j.biomaterials.2007.07.047.
Gersbach CA, Coyer SR, Le Doux JM, García AJ. Biomaterial-mediated retroviral gene transfer using self-assembled monolayers. Biomaterials. 2007 Dec;28(34):5121–7.
Gersbach, Charles A., et al. “Biomaterial-mediated retroviral gene transfer using self-assembled monolayers.Biomaterials, vol. 28, no. 34, Dec. 2007, pp. 5121–27. Epmc, doi:10.1016/j.biomaterials.2007.07.047.
Gersbach CA, Coyer SR, Le Doux JM, García AJ. Biomaterial-mediated retroviral gene transfer using self-assembled monolayers. Biomaterials. 2007 Dec;28(34):5121–5127.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

December 2007

Volume

28

Issue

34

Start / End Page

5121 / 5127

Related Subject Headings

  • Retroviridae
  • Polymers
  • Polylysine
  • Plasmids
  • NIH 3T3 Cells
  • Microscopy, Electron, Scanning
  • Mice
  • Genetic Therapy
  • Genetic Engineering
  • Gene Transfer Techniques