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Aminopyridines and sparteine as inhibitors of membrane potassium conductance: effects on Myxicola giant axons and the lobster neuromuscular junction.

Publication ,  Journal Article
Schauf, CL; Colton, CA; Colton, JS; Davis, FA
Published in: J Pharmacol Exp Ther
May 1976

The effects of the compounds 2-, 3- and 4-aminopyridine and sparteine on membrane conductance changes were examined using both voltage-clamped Myxicola axons and the lobster neuromuscular junction. In Myxicola axons, the aminopyridines very specifically inhibited the potassium conductance when applied at concentrations of 0.1 mM to 5 mM without any apparent effect of resting membrane potential. Concentrations in excess of 5 mM were needed to inhibit noticeably the sodium conductance. Potassium conductance-voltage curves were shifted in the depolarized direction along the voltage axis with no significant change in shape. There were only minor changes in the kinetics of potassium activation. In high potassium solutions, both inward and outward potassium currents were equally sensitive to the aminopyridines. Sparteine was, in general, found to be a more potent, but somewhat less specific, inhibitor of the potassium conductance. In contrast to the aminopyridines, sparteine was more effective when applied at basic pH and in addition tended to produce a noticeable degree of potassium inactivation. When applied to the lobster neuromuscular junction, 2-aminopyridine and sparteine dramatically increased the amplitude of both excitatory and inhibitory postjunctional potentials, with little or no change in resting potential, resting input conductance, reversal potential, or miniature end plate potential amplitude or frequency. Quantal content per fiber was increased by approximately a factor of 3 for the excitatory responses.

Duke Scholars

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

May 1976

Volume

197

Issue

2

Start / End Page

414 / 425

Location

United States

Related Subject Headings

  • Synapses
  • Sparteine
  • Pyridines
  • Potassium
  • Polychaeta
  • Pharmacology & Pharmacy
  • Neuromuscular Junction
  • Nephropidae
  • Membrane Potentials
  • In Vitro Techniques
 

Citation

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ICMJE
MLA
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Schauf, C. L., Colton, C. A., Colton, J. S., & Davis, F. A. (1976). Aminopyridines and sparteine as inhibitors of membrane potassium conductance: effects on Myxicola giant axons and the lobster neuromuscular junction. J Pharmacol Exp Ther, 197(2), 414–425.
Schauf, C. L., C. A. Colton, J. S. Colton, and F. A. Davis. “Aminopyridines and sparteine as inhibitors of membrane potassium conductance: effects on Myxicola giant axons and the lobster neuromuscular junction.J Pharmacol Exp Ther 197, no. 2 (May 1976): 414–25.

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

May 1976

Volume

197

Issue

2

Start / End Page

414 / 425

Location

United States

Related Subject Headings

  • Synapses
  • Sparteine
  • Pyridines
  • Potassium
  • Polychaeta
  • Pharmacology & Pharmacy
  • Neuromuscular Junction
  • Nephropidae
  • Membrane Potentials
  • In Vitro Techniques