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Comparative genomic hybridization analysis of astrocytomas: prognostic and diagnostic implications.

Publication ,  Journal Article
Wiltshire, RN; Herndon, JE; Lloyd, A; Friedman, HS; Bigner, DD; Bigner, SH; McLendon, RE
Published in: J Mol Diagn
August 2004

Astrocytoma is comprised of a group of common intracranial neoplasms that are classified into four grades based on the World Health Organization histological criteria and patient survival. To date, histological grade, patient age, and clinical performance, as reflected in the Karnofsky score, are the most reliable prognostic predictors. Recently, there has been a significant effort to identify additional prognostic markers using objective molecular genetic techniques. We believe that the identification of such markers will characterize new chromosomal loci important in astrocytoma progression and aid clinical diagnosis and prognosis. To this end, our laboratory used comparative genomic hybridization to identify DNA sequence copy number changes in 102 astrocytomas. Novel losses of 19p loci were detected in low-grade pilocytic astrocytomas and losses of loci on 9p, 10, and 22 along with gains on 7, 19, and 20 were detected in a significant proportion of high-grade astrocytomas. The Cox proportional hazards statistical modeling showed that the presence of +7q and -10q comparative genomic hybridization alterations significantly increased a patient's risk of dying, independent of histological grade. This investigation demonstrates the efficacy of comparative genomic hybridization for identifying tumor suppressor and oncogene loci in different astrocytic grades. The cumulative effect of these loci is an important consideration in their diagnostic and prognostic implications.

Duke Scholars

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Published In

J Mol Diagn

DOI

ISSN

1525-1578

Publication Date

August 2004

Volume

6

Issue

3

Start / End Page

166 / 179

Location

United States

Related Subject Headings

  • Prognosis
  • Pathology
  • Nucleic Acid Hybridization
  • Middle Aged
  • Male
  • Infant
  • Humans
  • Female
  • Chromosomes, Human
  • Chromosome Deletion
 

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Wiltshire, R. N., Herndon, J. E., Lloyd, A., Friedman, H. S., Bigner, D. D., Bigner, S. H., & McLendon, R. E. (2004). Comparative genomic hybridization analysis of astrocytomas: prognostic and diagnostic implications. J Mol Diagn, 6(3), 166–179. https://doi.org/10.1016/S1525-1578(10)60507-7
Wiltshire, Rodney N., James E. Herndon, Annie Lloyd, Henry S. Friedman, Darell D. Bigner, Sandra H. Bigner, and Roger E. McLendon. “Comparative genomic hybridization analysis of astrocytomas: prognostic and diagnostic implications.J Mol Diagn 6, no. 3 (August 2004): 166–79. https://doi.org/10.1016/S1525-1578(10)60507-7.
Wiltshire RN, Herndon JE, Lloyd A, Friedman HS, Bigner DD, Bigner SH, et al. Comparative genomic hybridization analysis of astrocytomas: prognostic and diagnostic implications. J Mol Diagn. 2004 Aug;6(3):166–79.
Wiltshire, Rodney N., et al. “Comparative genomic hybridization analysis of astrocytomas: prognostic and diagnostic implications.J Mol Diagn, vol. 6, no. 3, Aug. 2004, pp. 166–79. Pubmed, doi:10.1016/S1525-1578(10)60507-7.
Wiltshire RN, Herndon JE, Lloyd A, Friedman HS, Bigner DD, Bigner SH, McLendon RE. Comparative genomic hybridization analysis of astrocytomas: prognostic and diagnostic implications. J Mol Diagn. 2004 Aug;6(3):166–179.
Journal cover image

Published In

J Mol Diagn

DOI

ISSN

1525-1578

Publication Date

August 2004

Volume

6

Issue

3

Start / End Page

166 / 179

Location

United States

Related Subject Headings

  • Prognosis
  • Pathology
  • Nucleic Acid Hybridization
  • Middle Aged
  • Male
  • Infant
  • Humans
  • Female
  • Chromosomes, Human
  • Chromosome Deletion