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Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer.

Publication ,  Journal Article
Simon, SI; Nyunt, T; Florine-Casteel, K; Ritchie, K; Ting-Beall, HP; Evans, E; Needham, D
Published in: Annals of biomedical engineering
April 2007

A novel biointerface probe was implemented to study the deformability of the neutrophil membrane and cortical cytoskeleton. Piconewton scale forces are applied to the cell using an ultrasensitive and tunable force transducer comprised of an avidin-coated microsphere attached to a biotinylated and swollen red blood cell. Deformations of freshly isolated human neutrophils were observed on the stage of an inverted phase contrast microscope. Force versus probe indentation curves over a cycle of contact, indentation, and retraction revealed three distinct material responses. Small probe deformations (approximately 500 nm) tested over a range of rates (e.g. 100-500 nm/s) revealed predominantly an elastic response. An initial low-slope region in the force-indentation curves (approximately 0.005 pN/nm), typically extending 0.5-1.0 microm from the cell surface was interpreted as probe contact with microvilli extensions. Further deformation yielded a slope of 0.054+/-0.006 pN/nm, indicative of a stiffer cortical membrane. Disrupting cytoskeletal actin organization by pretreatment with cytochalasin D, reduced the slope by 40% to 0.033+/-0.007 pN/nm and introduced hysteresis in the recovery phase. Modeling the neutrophil as a liquid drop with constant surface tension yielded values of cortical tension of 0.035 pN/nm for resting and 0.02 pN/nm for cytochalasin-treated neutrophils. These data demonstrate the utility of the biointerface probe for measuring local surface compliance and microstructure of living cells.

Duke Scholars

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

April 2007

Volume

35

Issue

4

Start / End Page

595 / 604

Related Subject Headings

  • Surface Tension
  • Stress, Mechanical
  • Nucleic Acid Synthesis Inhibitors
  • Neutrophils
  • Models, Biological
  • Micromanipulation
  • Humans
  • Erythrocytes
  • Elasticity
  • Cytoskeleton
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Simon, S. I., Nyunt, T., Florine-Casteel, K., Ritchie, K., Ting-Beall, H. P., Evans, E., & Needham, D. (2007). Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer. Annals of Biomedical Engineering, 35(4), 595–604. https://doi.org/10.1007/s10439-007-9260-7
Simon, Scott I., Tun Nyunt, Kathryn Florine-Casteel, Ken Ritchie, H. P. Ting-Beall, Evan Evans, and David Needham. “Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer.Annals of Biomedical Engineering 35, no. 4 (April 2007): 595–604. https://doi.org/10.1007/s10439-007-9260-7.
Simon SI, Nyunt T, Florine-Casteel K, Ritchie K, Ting-Beall HP, Evans E, et al. Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer. Annals of biomedical engineering. 2007 Apr;35(4):595–604.
Simon, Scott I., et al. “Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer.Annals of Biomedical Engineering, vol. 35, no. 4, Apr. 2007, pp. 595–604. Epmc, doi:10.1007/s10439-007-9260-7.
Simon SI, Nyunt T, Florine-Casteel K, Ritchie K, Ting-Beall HP, Evans E, Needham D. Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer. Annals of biomedical engineering. 2007 Apr;35(4):595–604.
Journal cover image

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

April 2007

Volume

35

Issue

4

Start / End Page

595 / 604

Related Subject Headings

  • Surface Tension
  • Stress, Mechanical
  • Nucleic Acid Synthesis Inhibitors
  • Neutrophils
  • Models, Biological
  • Micromanipulation
  • Humans
  • Erythrocytes
  • Elasticity
  • Cytoskeleton