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Plasmonic detection of a model analyte in serum by a gold nanorod sensor.

Publication ,  Journal Article
Marinakos, SM; Chen, S; Chilkoti, A
Published in: Analytical chemistry
July 2007

We describe the fabrication of a label-free, chip-based biosensor based on the localized surface plasmon resonance (LSPR) of gold nanorods. Gold nanorods were chemisorbed onto a mercaptosilane-modified glass substrate, followed by conjugation of biotin to the nanorods. Streptavidin binding to biotin was monitored by the wavelength shift of the LSPR peak in the UV-vis extinction spectrum of the immobilized gold nanorods due to the change in local refractive index at the gold nanorod surface induced by streptavidin binding. The limit of detection of the sensor is 0.005 microg/mL (94 pM) in PBS and 1 microg/mL (19 nM) in serum, and the dynamic range spans 94 pM to 0.19 microM. The advantages of the nanorod-based sensor over an LSPR sensor that we had previously fabricated from gold nanospheres (Nath, N.; Chilkoti, A. Anal. Chem. 2002, 74, 504-509; J. Fluoresc. 2004, 14, 377-389; Anal. Chem. 2004, 76, 5370-5378) are the significantly lower detection limit and the internal self-reference that the signal of the nanorod sensor provides based on the measurement of peak wavelength shift.

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

July 2007

Volume

79

Issue

14

Start / End Page

5278 / 5283

Related Subject Headings

  • Surface Plasmon Resonance
  • Streptavidin
  • Serum
  • Sensitivity and Specificity
  • Nanotubes
  • Metal Nanoparticles
  • Humans
  • Gold
  • Glass
  • Biotin
 

Citation

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Marinakos, S. M., Chen, S., & Chilkoti, A. (2007). Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Analytical Chemistry, 79(14), 5278–5283. https://doi.org/10.1021/ac0706527
Marinakos, Stella M., Sihai Chen, and Ashutosh Chilkoti. “Plasmonic detection of a model analyte in serum by a gold nanorod sensor.Analytical Chemistry 79, no. 14 (July 2007): 5278–83. https://doi.org/10.1021/ac0706527.
Marinakos SM, Chen S, Chilkoti A. Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Analytical chemistry. 2007 Jul;79(14):5278–83.
Marinakos, Stella M., et al. “Plasmonic detection of a model analyte in serum by a gold nanorod sensor.Analytical Chemistry, vol. 79, no. 14, July 2007, pp. 5278–83. Epmc, doi:10.1021/ac0706527.
Marinakos SM, Chen S, Chilkoti A. Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Analytical chemistry. 2007 Jul;79(14):5278–5283.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

July 2007

Volume

79

Issue

14

Start / End Page

5278 / 5283

Related Subject Headings

  • Surface Plasmon Resonance
  • Streptavidin
  • Serum
  • Sensitivity and Specificity
  • Nanotubes
  • Metal Nanoparticles
  • Humans
  • Gold
  • Glass
  • Biotin