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In pursuit of zero: Polymer brushes that resist the adsorption of proteins

Publication ,  Journal Article
Hucknall, A; Rangarajan, S; Chilkoti, A
Published in: Advanced Materials
June 19, 2009

Protein resistant or "non-fouling" surfaces are of great interest for a variety of biomedical and biotechnology applications. This article briefly reviews the development of protein resistant surfaces, followed by recent research on a new methodology to fabricate non-fouling surfaces by surface-initiated polymerization. We show that polymer brushes synthesized by surfaceinitiated polymerization that present short oligo(ethylene glycol) side chains are exceptionally resistant to protein adsorption and cell adhesion. The importance of the protein and cell resistance conferred by these polymer brushes is illustrated by their use as substrates for the fabrication of antibody microarrays that exhibit femtomolar limits of detection in complex fluids such as serum and blood with relaxed requirements for intermediate wash steps. This example highlights the important point that the reduction in background noise afforded by protein-resistant surfaces can greatly simplify the development of ultrasensitive heterogeneous, surface-based clinical and proteomic assays with increased sensitivity and utility. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA,.

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Published In

Advanced Materials

DOI

ISSN

0935-9648

Publication Date

June 19, 2009

Volume

21

Issue

23

Start / End Page

2441 / 2446

Related Subject Headings

  • Nanoscience & Nanotechnology
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
  • 02 Physical Sciences
 

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Hucknall, A., Rangarajan, S., & Chilkoti, A. (2009). In pursuit of zero: Polymer brushes that resist the adsorption of proteins. Advanced Materials, 21(23), 2441–2446. https://doi.org/10.1002/adma.200900383
Hucknall, A., S. Rangarajan, and A. Chilkoti. “In pursuit of zero: Polymer brushes that resist the adsorption of proteins.” Advanced Materials 21, no. 23 (June 19, 2009): 2441–46. https://doi.org/10.1002/adma.200900383.
Hucknall A, Rangarajan S, Chilkoti A. In pursuit of zero: Polymer brushes that resist the adsorption of proteins. Advanced Materials. 2009 Jun 19;21(23):2441–6.
Hucknall, A., et al. “In pursuit of zero: Polymer brushes that resist the adsorption of proteins.” Advanced Materials, vol. 21, no. 23, June 2009, pp. 2441–46. Scopus, doi:10.1002/adma.200900383.
Hucknall A, Rangarajan S, Chilkoti A. In pursuit of zero: Polymer brushes that resist the adsorption of proteins. Advanced Materials. 2009 Jun 19;21(23):2441–2446.
Journal cover image

Published In

Advanced Materials

DOI

ISSN

0935-9648

Publication Date

June 19, 2009

Volume

21

Issue

23

Start / End Page

2441 / 2446

Related Subject Headings

  • Nanoscience & Nanotechnology
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
  • 02 Physical Sciences