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Surface-initiated enzymatic polymerization of DNA.

Publication ,  Journal Article
Chow, DC; Chilkoti, A
Published in: Langmuir : the ACS journal of surfaces and colloids
November 2007

We describe a technique to synthesize DNA homopolymers on a surface using surface-initiated enzymatic polymerization (SIEP) with terminal deoxynucleotidyl transferase (TdTase), an enzyme that repetitively adds mononucleotides to the 3'-end of oligonucleotides. The thickness of the synthesized DNA layer was found to depend on the deoxymononucleotide monomer, in the order of dATP > dTTP > dGTP approximately dCTP. In addition, the composition and the surface density of oligonucleotide initiators were also important in controlling the extent of DNA polymerization. The extension of single-stranded DNA chains by SIEP was further verified by their binding to antibodies specific to oligonucleotides. TdTase-mediated SIEP can also be used to grow spatially defined three-dimensional DNA structures by soft lithography and is a new tool for bioinspired fabrication at the micro- and nanoscale.

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Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

November 2007

Volume

23

Issue

23

Start / End Page

11712 / 11717

Related Subject Headings

  • Time Factors
  • Thymine Nucleotides
  • Surface Properties
  • Surface Plasmon Resonance
  • Spectrometry, X-Ray Emission
  • Polydeoxyribonucleotides
  • Oligonucleotides
  • Nanotechnology
  • Microscopy, Atomic Force
  • Deoxyguanine Nucleotides
 

Citation

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ICMJE
MLA
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Chow, D. C., & Chilkoti, A. (2007). Surface-initiated enzymatic polymerization of DNA. Langmuir : The ACS Journal of Surfaces and Colloids, 23(23), 11712–11717. https://doi.org/10.1021/la701630g
Chow, Dominic C., and Ashutosh Chilkoti. “Surface-initiated enzymatic polymerization of DNA.Langmuir : The ACS Journal of Surfaces and Colloids 23, no. 23 (November 2007): 11712–17. https://doi.org/10.1021/la701630g.
Chow DC, Chilkoti A. Surface-initiated enzymatic polymerization of DNA. Langmuir : the ACS journal of surfaces and colloids. 2007 Nov;23(23):11712–7.
Chow, Dominic C., and Ashutosh Chilkoti. “Surface-initiated enzymatic polymerization of DNA.Langmuir : The ACS Journal of Surfaces and Colloids, vol. 23, no. 23, Nov. 2007, pp. 11712–17. Epmc, doi:10.1021/la701630g.
Chow DC, Chilkoti A. Surface-initiated enzymatic polymerization of DNA. Langmuir : the ACS journal of surfaces and colloids. 2007 Nov;23(23):11712–11717.
Journal cover image

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

November 2007

Volume

23

Issue

23

Start / End Page

11712 / 11717

Related Subject Headings

  • Time Factors
  • Thymine Nucleotides
  • Surface Properties
  • Surface Plasmon Resonance
  • Spectrometry, X-Ray Emission
  • Polydeoxyribonucleotides
  • Oligonucleotides
  • Nanotechnology
  • Microscopy, Atomic Force
  • Deoxyguanine Nucleotides