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Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair.

Publication ,  Journal Article
McHale, MK; Setton, LA; Chilkoti, A
Published in: Tissue engineering
November 2005

Genetically engineered elastin-like polypeptide (ELP) hydrogels offer unique promise as scaffolds for cartilage tissue engineering because of the potential to promote chondrogenesis and to control mechanical properties. In this study, we designed and synthesized ELPs capable of undergoing enzyme-initiated gelation via tissue transglutaminase, with the ultimate goal of creating an injectable, in situ cross-linking scaffold to promote functional cartilage repair. Addition of the enzyme promoted ELP gel formation and chondrocyte encapsulation in a biocompatible process, which resulted in cartilage matrix synthesis in vitro and the potential to contribute to cartilage mechanical function in vivo. A significant increase in the accumulation of sulfated glycosaminoglycans was observed, and histological sections revealed the accumulation of a cartilaginous matrix rich in type II collagen and lacking in type I collagen, indicative of hyaline cartilage formation. These results provide evidence of chondrocytic phenotype maintenance for cells in the ELP hydrogels in vitro. In addition, the dynamic shear moduli of ELP hydrogels seeded with chondrocytes increased from 0.28 to 1.7 kPa during a 4-week culture period. This increase in the mechanical integrity of cross-linked ELP hydrogels suggests restructuring of the ELP matrix by deposition of functional cartilage extracellular matrix components.

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Published In

Tissue engineering

DOI

EISSN

1557-8690

ISSN

1076-3279

Publication Date

November 2005

Volume

11

Issue

11-12

Start / End Page

1768 / 1779

Related Subject Headings

  • Transglutaminases
  • Tissue Engineering
  • Swine
  • Recombinant Proteins
  • Hydrogels
  • Humans
  • Evaluation Studies as Topic
  • Elastin
  • Collagen Type I
  • Chondrocytes
 

Citation

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ICMJE
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McHale, M. K., Setton, L. A., & Chilkoti, A. (2005). Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. Tissue Engineering, 11(11–12), 1768–1779. https://doi.org/10.1089/ten.2005.11.1768
McHale, Melissa K., Lori A. Setton, and Ashutosh Chilkoti. “Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair.Tissue Engineering 11, no. 11–12 (November 2005): 1768–79. https://doi.org/10.1089/ten.2005.11.1768.
McHale MK, Setton LA, Chilkoti A. Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. Tissue engineering. 2005 Nov;11(11–12):1768–79.
McHale, Melissa K., et al. “Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair.Tissue Engineering, vol. 11, no. 11–12, Nov. 2005, pp. 1768–79. Epmc, doi:10.1089/ten.2005.11.1768.
McHale MK, Setton LA, Chilkoti A. Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. Tissue engineering. 2005 Nov;11(11–12):1768–1779.

Published In

Tissue engineering

DOI

EISSN

1557-8690

ISSN

1076-3279

Publication Date

November 2005

Volume

11

Issue

11-12

Start / End Page

1768 / 1779

Related Subject Headings

  • Transglutaminases
  • Tissue Engineering
  • Swine
  • Recombinant Proteins
  • Hydrogels
  • Humans
  • Evaluation Studies as Topic
  • Elastin
  • Collagen Type I
  • Chondrocytes