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Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression.

Publication ,  Journal Article
Hyun, J; Chen, J; Setton, LA; Chilkoti, A
Published in: Biomaterials
March 2006

We describe the fabrication of deformable microstructures by low-pressure-soft-microembossing (muSEmb) that provides in vitro experimental "test-beds" to investigate the interplay of mechanical and chemical stimuli on cell behavior in a highly controlled environment. Soft microembossing exploits the softness and plasticity of parafilm to fabricate microstructures by pressing a silicon master or an elastomeric poly(dimethylsiloxane) stamp into the parafilm. We demonstrate that a protein-resistant comb polymer can be printed into the raised features of the embossed microstructures, which imparts protein, and hence cell resistance to those regions of the microstructures. These two features of our fabrication methodology-microembossing followed by spatially selective transfer of a nonfouling polymer-forms the core of our strategy to pattern cells within the parafilm microstructures, such that the cells are confined within bottoms of the microstructures. Cell culture experiments demonstrated the preferential cell attachment of NIH 3T3 fibroblasts to the fibronectin (FN) micropatterns by immunofluorescence microscopy. The actin cytoskeleton realigned along the axis of applied mechanical stress, and stretched cells showed altered gene expression of cytoskeletal and matrix proteins in response to mechanical deformation. The use of parafilm as a substrate and muSEmb as a fabrication method provides a simple and widely accessible methodology to investigate cellular behavior under well-defined conditions of plastic deformation and surface ligand density.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

March 2006

Volume

27

Issue

8

Start / End Page

1444 / 1451

Related Subject Headings

  • Swine
  • Silicones
  • Phenotype
  • Paraffin
  • NIH 3T3 Cells
  • Microscopy, Phase-Contrast
  • Microscopy, Confocal
  • Mice
  • Materials Testing
  • Gene Expression Profiling
 

Citation

APA
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ICMJE
MLA
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Hyun, J., Chen, J., Setton, L. A., & Chilkoti, A. (2006). Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression. Biomaterials, 27(8), 1444–1451. https://doi.org/10.1016/j.biomaterials.2005.08.018
Hyun, Jinho, Jun Chen, Lori A. Setton, and Ashutosh Chilkoti. “Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression.Biomaterials 27, no. 8 (March 2006): 1444–51. https://doi.org/10.1016/j.biomaterials.2005.08.018.
Hyun, Jinho, et al. “Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression.Biomaterials, vol. 27, no. 8, Mar. 2006, pp. 1444–51. Epmc, doi:10.1016/j.biomaterials.2005.08.018.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

March 2006

Volume

27

Issue

8

Start / End Page

1444 / 1451

Related Subject Headings

  • Swine
  • Silicones
  • Phenotype
  • Paraffin
  • NIH 3T3 Cells
  • Microscopy, Phase-Contrast
  • Microscopy, Confocal
  • Mice
  • Materials Testing
  • Gene Expression Profiling