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Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney.

Publication ,  Journal Article
Hilgers, KF; Nagaraj, SK; Karginova, EA; Kazakova, IG; Chevalier, RL; Carey, RM; Pentz, ES; Gomez, RA
Published in: Kidney Int
November 1998

BACKGROUND: We aimed to identify genes with kidney specific, developmentally regulated expression. Here we report the cDNA sequence and expression pattern of KS, a novel kidney-specific rat gene. METHODS: A partial cDNA was identified by differential display polymerase chain reaction (PCR) of a renal cell fraction enriched for proximal tubular and renin-expressing cells. Using the partial cDNA as a probe, a rat kidney cDNA library was screened. The full-length KS sequence was obtained by PCR amplification of cDNA ends. The expression pattern of KS was investigated by Northern blot. RNA was extracted from several organs of newborn and adult rats, as well as from the kidneys of rats with altered tubular function, that is, rats that had undergone unilateral nephrectomy, unilateral ureteral obstruction, neonatal losartan treatment, and the appropriate control animals. The expression of KS was also investigated in the kidneys of rats with spontaneous or renovascular hypertension. RESULTS: The KS cDNA (2426 bp) contained one open reading frame encoding a predicted 572 amino acid protein. The derived peptide sequence displayed approximately 70% similarity to the hypertension-related SA gene product and approximately 50% similarity to prokaryotic and eukaryotic acetyl-CoA synthases (EC 6. 2.1.1). KS was expressed in the kidney and not in any other organ assayed. KS RNA was not detected in fetal and newborn rat kidney but became apparent after one week of postnatal life. Gene expression was downregulated in rat models of altered tubular function. KS expression was decreased in spontaneously hypertensive rats but not in renovascular hypertension. CONCLUSION: KS, a novel rat gene, exhibits a unique tissue-specific expression exclusively in mature kidneys. The data suggest KS may encode an adenosine monophosphate binding enzyme.

Duke Scholars

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Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

November 1998

Volume

54

Issue

5

Start / End Page

1444 / 1454

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Rats, Sprague-Dawley
  • Rats, Inbred WKY
  • Rats, Inbred SHR
  • Rats
  • Proteins
  • Organ Specificity
  • Molecular Sequence Data
  • Kidney
  • Gene Expression Regulation
 

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Hilgers, K. F., Nagaraj, S. K., Karginova, E. A., Kazakova, I. G., Chevalier, R. L., Carey, R. M., … Gomez, R. A. (1998). Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney. Kidney Int, 54(5), 1444–1454. https://doi.org/10.1046/j.1523-1755.1998.00143.x
Hilgers, K. F., S. K. Nagaraj, E. A. Karginova, I. G. Kazakova, R. L. Chevalier, R. M. Carey, E. S. Pentz, and R. A. Gomez. “Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney.Kidney Int 54, no. 5 (November 1998): 1444–54. https://doi.org/10.1046/j.1523-1755.1998.00143.x.
Hilgers KF, Nagaraj SK, Karginova EA, Kazakova IG, Chevalier RL, Carey RM, et al. Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney. Kidney Int. 1998 Nov;54(5):1444–54.
Hilgers, K. F., et al. “Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney.Kidney Int, vol. 54, no. 5, Nov. 1998, pp. 1444–54. Pubmed, doi:10.1046/j.1523-1755.1998.00143.x.
Hilgers KF, Nagaraj SK, Karginova EA, Kazakova IG, Chevalier RL, Carey RM, Pentz ES, Gomez RA. Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney. Kidney Int. 1998 Nov;54(5):1444–1454.
Journal cover image

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

November 1998

Volume

54

Issue

5

Start / End Page

1444 / 1454

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Rats, Sprague-Dawley
  • Rats, Inbred WKY
  • Rats, Inbred SHR
  • Rats
  • Proteins
  • Organ Specificity
  • Molecular Sequence Data
  • Kidney
  • Gene Expression Regulation